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| [[Image:1csl.jpg|left|200px]]<br /><applet load="1csl" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1csl, resolution 1.60Å" />
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| '''CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE'''<br />
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| ==Overview== | | ==CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE== |
| The crystal structure of a 28 nt RNA fragment containing the human immunodeficiency virus type 1 (HIV-1) Rev response element high affinity binding site for Rev protein has been solved at 1.6 A resolution. The overall structure of the RRE helix is greatly distorted from A-form geometry by the presence of two purine-purine base-pairs and two single nucleotide bulges. G48 and G71 form a Hoogsteen-type asymmetric base-pair with G71 adopting a syn conformation. The non-canonical regions in the unliganded Rev response element molecule narrow the major groove width with respect to standard A-RNA. The Rev response element structure observed here represents a closed form of the Rev binding site and differs from conformations of the RNA observed previously by solution NMR studies.
| | <StructureSection load='1csl' size='340' side='right'caption='[[1csl]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1csl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CSL FirstGlance]. <br> |
| 1CSL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSL OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1csl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1csl OCA], [https://pdbe.org/1csl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1csl RCSB], [https://www.ebi.ac.uk/pdbsum/1csl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1csl ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| The structure of the HIV-1 RRE high affinity rev binding site at 1.6 A resolution., Ippolito JA, Steitz TA, J Mol Biol. 2000 Jan 28;295(4):711-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10656783 10656783]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Ippolito, J A.]]
| | [[Category: Large Structures]] |
| [[Category: Steitz, T A.]] | | [[Category: Ippolito JA]] |
| [[Category: hiv-1]] | | [[Category: Steitz TA]] |
| [[Category: rre high affinity site]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:09:20 2008''
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