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[[Image:1ips.gif|left|200px]]<br />
<applet load="1ips" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ips, resolution 2.5&Aring;" />
'''ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MANGANESE COMPLEX)'''<br />


==Overview==
==ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MANGANESE COMPLEX)==
Penicillin antibiotics are all produced from fermentation-derived, penicillins because their chemical synthesis is not commercially viable., The key step in penicillin biosynthesis, in which both the beta-lactam and, thiazolidine rings of the nucleus are created, is mediated by, isopenicillin N synthase (IPNS), which binds ferrous iron and uses, dioxygen as a cosubstrate. In a unique enzymatic step, with no chemical, precedent, IPNS catalyses the transfer of four hydrogen atoms from its, tripeptide substrate to dioxygen forming, in a single reaction, the, complete bicyclic nucleus of the penicillins. We now report the structure, of IPNS complexed with manganese, which reveals the active site is, unusually buried within a 'jelly-roll' motif and lined by hydrophobic, residues, and suggest ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7791906 (full description)]]
<StructureSection load='1ips' size='340' side='right'caption='[[1ips]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ips]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IPS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ips FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ips OCA], [https://pdbe.org/1ips PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ips RCSB], [https://www.ebi.ac.uk/pdbsum/1ips PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ips ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IPNA_EMENI IPNA_EMENI] Isopenicillin N synthase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic (PubMed:3319778, PubMed:11755401). IpnA catalyzes the cyclization of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) to form isopenicillin N (IPN) that contains the beta-lactam nucleus (PubMed:3319778, PubMed:11755401, PubMed:28703303). The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS acvA. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase penDE to yield penicillin in the peroxisomal matrix (By similarity).[UniProtKB:P08703]<ref>PMID:11755401</ref> <ref>PMID:28703303</ref> <ref>PMID:3319778</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ip/1ips_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ips ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1IPS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: SA and SB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IPS OCA]].
*[[Isopenicillin N synthase|Isopenicillin N synthase]]
 
== References ==
==Reference==
<references/>
Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes., Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE, Nature. 1995 Jun 22;375(6533):700-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7791906 7791906]
__TOC__
[[Category: Emericella nidulans]]
</StructureSection>
[[Category: Single protein]]
[[Category: Aspergillus nidulans]]
[[Category: Andersson, I.]]
[[Category: Large Structures]]
[[Category: Baldwin, J.E.]]
[[Category: Andersson I]]
[[Category: Barton, G.J.]]
[[Category: Baldwin JE]]
[[Category: Clifton, I.J.]]
[[Category: Barton GJ]]
[[Category: Fulop, V.]]
[[Category: Clifton IJ]]
[[Category: Hajdu, J.]]
[[Category: Fulop V]]
[[Category: Harlos, K.]]
[[Category: Hajdu J]]
[[Category: Roach, P.L.]]
[[Category: Harlos K]]
[[Category: Schofield, C.J.]]
[[Category: Roach PL]]
[[Category: MN]]
[[Category: Schofield CJ]]
[[Category: antibiotic biosynthesis]]
[[Category: b-lactam antibiotic]]
[[Category: oxidoreductase]]
[[Category: oxygenase]]
[[Category: penicillin biosynthesis]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:47:51 2007''
Retrieved from "https://proteopedia.org/1ips"