1d4b: Difference between revisions

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[[Image:1d4b.gif|left|200px]]<br /><applet load="1d4b" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1d4b" />
'''CIDE-N DOMAIN OF HUMAN CIDE-B'''<br />


==Overview==
==CIDE-N DOMAIN OF HUMAN CIDE-B==
<StructureSection load='1d4b' size='340' side='right'caption='[[1d4b]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1d4b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D4B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d4b OCA], [https://pdbe.org/1d4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d4b RCSB], [https://www.ebi.ac.uk/pdbsum/1d4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d4b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CIDEB_HUMAN CIDEB_HUMAN] Activates apoptosis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d4/1d4b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d4b ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Apoptotic DNA fragmentation and chromatin condensation are mediated by the caspase-activated DFF40/ CAD nuclease, which is chaperoned and inhibited by DFF45/ICAD. CIDE proteins share a homologous regulatory CIDE-N domain with DFF40/CAD and DFF45/ ICAD. Here we report the solution structure of CIDE-N of human CIDE-B. We show that the CIDE-N of CIDE-B interacts with CIDE-N domains of both DFF40 and DFF45. The binding epitopes are similar and map to a highly charged bipolar surface region of CIDE-B. Furthermore, we demonstrate that the CIDE-N of CIDE-B regulates enzymatic activity of the DFF40/ DFF45 complex in vitro. Based on these results and mutagenesis data, we propose a model for the CIDE-N/ CIDE-N complex and discuss the role of this novel bipolar interaction in mediating downstream events of apoptosis.
Apoptotic DNA fragmentation and chromatin condensation are mediated by the caspase-activated DFF40/ CAD nuclease, which is chaperoned and inhibited by DFF45/ICAD. CIDE proteins share a homologous regulatory CIDE-N domain with DFF40/CAD and DFF45/ ICAD. Here we report the solution structure of CIDE-N of human CIDE-B. We show that the CIDE-N of CIDE-B interacts with CIDE-N domains of both DFF40 and DFF45. The binding epitopes are similar and map to a highly charged bipolar surface region of CIDE-B. Furthermore, we demonstrate that the CIDE-N of CIDE-B regulates enzymatic activity of the DFF40/ DFF45 complex in vitro. Based on these results and mutagenesis data, we propose a model for the CIDE-N/ CIDE-N complex and discuss the role of this novel bipolar interaction in mediating downstream events of apoptosis.


==About this Structure==
Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis.,Lugovskoy AA, Zhou P, Chou JJ, McCarty JS, Li P, Wagner G Cell. 1999 Dec 23;99(7):747-55. PMID:10619428<ref>PMID:10619428</ref>
1D4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D4B OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis., Lugovskoy AA, Zhou P, Chou JJ, McCarty JS, Li P, Wagner G, Cell. 1999 Dec 23;99(7):747-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10619428 10619428]
</div>
<div class="pdbe-citations 1d4b" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chou, J.]]
[[Category: Chou J]]
[[Category: Li, P.]]
[[Category: Li P]]
[[Category: Lugovskoy, A.]]
[[Category: Lugovskoy A]]
[[Category: McCarty, J.]]
[[Category: McCarty J]]
[[Category: Wagner, G.]]
[[Category: Wagner G]]
[[Category: Zhou, P.]]
[[Category: Zhou P]]
[[Category: alpha/beta roll]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:49 2008''

Latest revision as of 08:23, 22 May 2024

CIDE-N DOMAIN OF HUMAN CIDE-B

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