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[[Image:3qom.png|left|200px]]


{{STRUCTURE_3qom| PDB=3qom | SCENE= }}
==Crystal structure of 6-phospho-beta-glucosidase from Lactobacillus plantarum==
<StructureSection load='3qom' size='340' side='right'caption='[[3qom]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3qom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QOM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.498&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qom OCA], [https://pdbe.org/3qom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qom RCSB], [https://www.ebi.ac.uk/pdbsum/3qom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qom ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F9UU25_LACPL F9UU25_LACPL]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In lactic acid bacteria and other bacteria, carbohydrate uptake is mostly governed by phosphoenolpyruvate-dependent phosphotransferase systems (PTSs). PTS-dependent translocation through the cell membrane is coupled with phosphorylation of the incoming sugar. After translocation through the bacterial membrane, the beta-glycosidic bond in 6'-P-beta-glucoside is cleaved, releasing 6-P-beta-glucose and the respective aglycon. This reaction is catalyzed by 6-P-beta-glucosidases, which belong to two glycoside hydrolase (GH) families: GH1 and GH4. Here, the high-resolution crystal structures of GH1 6-P-beta-glucosidases from Lactobacillus plantarum (LpPbg1) and Streptococcus mutans (SmBgl) and their complexes with ligands are reported. Both enzymes show hydrolytic activity towards 6'-P-beta-glucosides. The LpPbg1 structure has been determined in an apo form as well as in a complex with phosphate and a glucose molecule corresponding to the aglycon molecule. The S. mutans homolog contains a sulfate ion in the phosphate-dedicated subcavity. SmBgl was also crystallized in the presence of the reaction product 6-P-beta-glucose. For a mutated variant of the S. mutans enzyme (E375Q), the structure of a 6'-P-salicin complex has also been determined. The presence of natural ligands enabled the definition of the structural elements that are responsible for substrate recognition during catalysis.


===Crystal structure of 6-phospho-beta-glucosidase from Lactobacillus plantarum===
GH1-family 6-P-beta-glucosidases from human microbiome lactic acid bacteria.,Michalska K, Tan K, Li H, Hatzos-Skintges C, Bearden J, Babnigg G, Joachimiak A Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):451-63. doi:, 10.1107/S0907444912049608. Epub 2013 Feb 16. PMID:23519420<ref>PMID:23519420</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
[[3qom]] is a 1 chain structure of [[Beta-glucosidase]] with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QOM OCA].
<div class="pdbe-citations 3qom" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Beta-glucosidase|Beta-glucosidase]]
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
[[Category: 6-phospho-beta-glucosidase]]
== References ==
[[Category: Lactobacillus plantarum]]
<references/>
[[Category: Bearden, J.]]
__TOC__
[[Category: Hatzos-Skintges, C.]]
</StructureSection>
[[Category: Joachimiak, A.]]
[[Category: Lactiplantibacillus plantarum]]
[[Category: Kohler, M.]]
[[Category: Large Structures]]
[[Category: MCSG, Midwest Center for Structural Genomics.]]
[[Category: Bearden J]]
[[Category: Michalska, K.]]
[[Category: Hatzos-Skintges C]]
[[Category: Glycoside hydrolase]]
[[Category: Joachimiak A]]
[[Category: Hydrolase]]
[[Category: Kohler M]]
[[Category: Mcsg]]
[[Category: Michalska K]]
[[Category: Midwest center for structural genomic]]
[[Category: Psi-biology]]
[[Category: Structural genomic]]
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