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[[Image:1dv5.jpg|left|200px]]<br /><applet load="1dv5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1dv5" />
'''TERTIARY STRUCTURE OF APO-D-ALANYL CARRIER PROTEIN'''<br />


==Overview==
==TERTIARY STRUCTURE OF APO-D-ALANYL CARRIER PROTEIN==
The D-alanylation of lipoteichoic acid (LTA) allows the Gram-positive organism to modulate its surface charge, regulate ligand binding, and control the electromechanical properties of the cell wall. The incorporation of D-alanine into LTA requires the D-alanine:D-alanyl carrier protein ligase (AMP-forming) (Dcl) and the carrier protein (Dcp). The high-resolution solution structure of the 81-residue (8.9 kDa) Dcp has been determined by multidimensional heteronuclear NMR. An ensemble of 30 structures was calculated using the torsion angle dynamics approach of DYANA. These calculations utilized 3288 NOEs containing 1582 unique nontrivial NOE distance constraints. Superposition of residues 4-81 on the mean structure yields average atomic rmsd values of 0.43 +/- 0.08 and 0.86 +/- 0.09 A for backbone and non-hydrogen atoms, respectively. The solution structure is composed of three alpha-helices in a bundle with additional short 3(10)- and alpha-helices in intervening loops. Comparisons of the three-dimensional structure with the acyl carrier proteins involved in fatty acid, polyketide, and nonribosomal peptide syntheses support the conclusion that Dcp is a homologue in this family. While there is conservation of the three-helix bundle fold, Dcp has a higher enthalpy of unfolding and no apparent divalent metal binding site(s), features that distinguish it from the fatty acid synthase acyl carrier protein of Escherichia coli. This three-dimensional structure also provides insights into the D-alanine ligation site recognized by Dcl, as well as the site which may bind the poly(glycerophosphate) acceptor moiety of membrane-associated LTA.
<StructureSection load='1dv5' size='340' side='right'caption='[[1dv5]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1dv5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_casei Lacticaseibacillus casei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DV5 FirstGlance]. <br>
1DV5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dv5 OCA], [https://pdbe.org/1dv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dv5 RCSB], [https://www.ebi.ac.uk/pdbsum/1dv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dv5 ProSAT]</span></td></tr>
==Reference==
</table>
Biosynthesis of D-alanyl-lipoteichoic acid: the tertiary structure of apo-D-alanyl carrier protein., Volkman BF, Zhang Q, Debabov DV, Rivera E, Kresheck GC, Neuhaus FC, Biochemistry. 2001 Jul 10;40(27):7964-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11434765 11434765]
== Function ==
[[Category: Lactobacillus casei]]
[https://www.uniprot.org/uniprot/DLTC_LACRH DLTC_LACRH] Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Activated D-alanyl-Dcp donates its D-alanyl substituent to membrane-associated LTA.
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Debabov, D V.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kresheck, G C.]]
Check<jmol>
[[Category: Neuhaus, F C.]]
  <jmolCheckbox>
[[Category: Rivera, E.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dv/1dv5_consurf.spt"</scriptWhenChecked>
[[Category: Volkman, B F.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Zhang, Q.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: 3-helix bundle]]
  </jmolCheckbox>
 
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dv5 ConSurf].
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:49 2008''
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Lacticaseibacillus casei]]
[[Category: Large Structures]]
[[Category: Debabov DV]]
[[Category: Kresheck GC]]
[[Category: Neuhaus FC]]
[[Category: Rivera E]]
[[Category: Volkman BF]]
[[Category: Zhang Q]]

Latest revision as of 09:54, 20 March 2024

TERTIARY STRUCTURE OF APO-D-ALANYL CARRIER PROTEIN

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