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[[Image:1el4.gif|left|200px]]<br /><applet load="1el4" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1el4, resolution 1.73&Aring;" />
'''STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS'''<br />


==Overview==
==STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS==
The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 A resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination.
<StructureSection load='1el4' size='340' side='right'caption='[[1el4]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1el4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EL4 FirstGlance]. <br>
1EL4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CTZ:'>CTZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL4 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTZ:C2-HYDROXY-COELENTERAZINE'>CTZ</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1el4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1el4 OCA], [https://pdbe.org/1el4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1el4 RCSB], [https://www.ebi.ac.uk/pdbsum/1el4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1el4 ProSAT]</span></td></tr>
Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure., Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC, Protein Sci. 2000 Nov;9(11):2085-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11152120 11152120]
</table>
== Function ==
[https://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1el4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1el4 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Obelia longissima]]
[[Category: Obelia longissima]]
[[Category: Single protein]]
[[Category: Lee J]]
[[Category: Lee, J.]]
[[Category: Liu ZJ]]
[[Category: Liu, Z J.]]
[[Category: Rose J]]
[[Category: Rose, J.]]
[[Category: Vysotski ES]]
[[Category: Vysotski, E S.]]
[[Category: Wang BC]]
[[Category: Wang, B C.]]
[[Category: CL]]
[[Category: CTZ]]
[[Category: bioluminescence]]
[[Category: calcium]]
[[Category: obelin]]
[[Category: photoprotein]]
[[Category: sulfur anomalous scattering]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:53 2008''

Latest revision as of 07:04, 7 February 2024

STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS

1el4, resolution 1.73Å

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