1elu: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1elu.gif|left|200px]]<br /><applet load="1elu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1elu, resolution 1.55&Aring;" />
'''COMPLEX BETWEEN THE CYSTINE C-S LYASE C-DES AND ITS REACTION PRODUCT CYSTEINE PERSULFIDE.'''<br />


==Overview==
==COMPLEX BETWEEN THE CYSTINE C-S LYASE C-DES AND ITS REACTION PRODUCT CYSTEINE PERSULFIDE.==
FeS clusters are versatile cofactors of a variety of proteins, but the mechanisms of their biosynthesis are still unknown. The cystine C-S lyase from Synechocystis has been identified as a participant in ferredoxin FeS cluster formation. Herein, we report on the crystal structure of the lyase and of a complex with the reaction products of cystine cleavage at 1.8- and 1.55-A resolution, respectively. The sulfur-containing product was unequivocally identified as cysteine persulfide. The reactive persulfide group is fixed by a hydrogen bond to His-114 in the center of a hydrophobic pocket and is thereby shielded from the solvent. Binding and stabilization of the cysteine persulfide represent an alternative to the generation of a protein-bound persulfide by NifS-like proteins and point to the general importance of persulfidic compounds for FeS cluster assembly.
<StructureSection load='1elu' size='340' side='right'caption='[[1elu]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1elu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6714 Synechocystis sp. PCC 6714]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELU FirstGlance]. <br>
1ELU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=PDA:'>PDA</scene> and <scene name='pdbligand=CSS:'>CSS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PDA:2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL)-AMINO]-PROPIONIC+ACID'>PDA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elu OCA], [https://pdbe.org/1elu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elu RCSB], [https://www.ebi.ac.uk/pdbsum/1elu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elu ProSAT]</span></td></tr>
Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis., Clausen T, Kaiser JT, Steegborn C, Huber R, Kessler D, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3856-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10760256 10760256]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Synechocystis sp.]]
[https://www.uniprot.org/uniprot/Q9ZHG9_SYNY4 Q9ZHG9_SYNY4]  
[[Category: Clausen, T.]]
== Evolutionary Conservation ==
[[Category: Huber, R.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kaiser, J T.]]
Check<jmol>
[[Category: Kessler, D.]]
  <jmolCheckbox>
[[Category: Steegborn, C.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elu_consurf.spt"</scriptWhenChecked>
[[Category: CSS]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: K]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: PDA]]
  </jmolCheckbox>
[[Category: aminoacrylate]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elu ConSurf].
[[Category: enzyme-product complex]]
<div style="clear:both"></div>
[[Category: fes cluster biosynthesis]]
__TOC__
[[Category: pyridoxal 5'-phosphate]]
</StructureSection>
[[Category: thiocysteine]]
[[Category: Large Structures]]
 
[[Category: Synechocystis sp. PCC 6714]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:09 2008''
[[Category: Clausen T]]
[[Category: Huber R]]
[[Category: Kaiser JT]]
[[Category: Kessler D]]
[[Category: Steegborn C]]

Latest revision as of 07:04, 7 February 2024

COMPLEX BETWEEN THE CYSTINE C-S LYASE C-DES AND ITS REACTION PRODUCT CYSTEINE PERSULFIDE.

1elu, resolution 1.55Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1elu&oldid=4042694"