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[[Image:3gua.png|left|200px]]


{{STRUCTURE_3gua| PDB=3gua | SCENE= }}
==Sulfates bound in the vestibule of AChBP==
<StructureSection load='3gua' size='340' side='right'caption='[[3gua]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3gua]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gua OCA], [https://pdbe.org/3gua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gua RCSB], [https://www.ebi.ac.uk/pdbsum/3gua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gua ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3gua_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gua ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Neurotransmitter binding to Cys-loop receptors promotes a prodigious transmembrane flux of several million ions/s, but to date, structural determinants of ion flux have been identified flanking the membrane-spanning region. Using x-ray crystallography, sequence analysis, and single-channel recording, we identified a novel determinant of ion conductance near the point of entry of permeant ions. Co-crystallization of acetylcholine-binding protein with sulfate anions revealed coordination of SO4(2-) with a ring of lysines at a position equivalent to 24 A above the lipid membrane in homologous Cys-loop receptors. Analysis of multiple sequence alignments revealed that residues equivalent to the ring of lysines are negatively charged in cation-selective receptors but are positively charged in anion-selective receptors. Charge reversal of side chains at homologous positions in the nicotinic receptor from the motor end plate decreases unitary conductance up to 80%. Selectivity filters stemming from transmembrane alpha-helices have similar pore diameters and compositions of amino acids. These findings establish that when the channel opens under a physiological electrochemical gradient, permeant ions are initially stabilized within the extracellular vestibule of Cys-loop receptors, and this stabilization is a major determinant of ion conductance.


===Sulfates bound in the vestibule of AChBP===
An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance.,Hansen SB, Wang HL, Taylor P, Sine SM J Biol Chem. 2008 Dec 26;283(52):36066-70. Epub 2008 Oct 21. PMID:18940802<ref>PMID:18940802</ref>


{{ABSTRACT_PUBMED_18940802}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3gua" style="background-color:#fffaf0;"></div>
[[3gua]] is a 10 chain structure of [[Acetylcholine binding protein]] with sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUA OCA].


==See Also==
==See Also==
*[[Acetylcholine binding protein|Acetylcholine binding protein]]
*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:018940802</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
[[Category: Hansen, S B.]]
[[Category: Large Structures]]
[[Category: Taylor, P.]]
[[Category: Hansen SB]]
[[Category: Choline-binding protein]]
[[Category: Taylor P]]
[[Category: Ion channel]]
[[Category: Ion filter]]
[[Category: Ion selectivity]]
[[Category: Receptor]]
Retrieved from "https://proteopedia.org/3gua"