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[[Image:2k2a.png|left|200px]]


{{STRUCTURE_2k2a| PDB=2k2a | SCENE= }}
==Solution Structure of the Apo C terminal domain of Lethocerus troponin C isoform F1==
<StructureSection load='2k2a' size='340' side='right'caption='[[2k2a]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2k2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K2A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k2a OCA], [https://pdbe.org/2k2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k2a RCSB], [https://www.ebi.ac.uk/pdbsum/2k2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k2a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q868D4_9HEMI Q868D4_9HEMI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/2k2a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k2a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Muscle contraction is activated by two distinct mechanisms. One depends on the calcium influx, and the other is calcium-independent and activated by mechanical stress. A prototypical example of stretch activation is observed in insect muscles. In Lethocerus, a model system ideally suited for studying stretch activation, the two mechanisms seem to be under the control of different isoforms of troponin C (TnC), F1 and F2, which are responsible for stretch and calcium-dependent regulation, respectively. We have previously shown that F1 TnC is a typical collapsed dumbbell EF-hand protein that accommodates one calcium ion in its fourth EF-hand. When calcium loaded, the C-terminal domain of F1 TnC is in an open conformation which allows binding to troponin I. We have determined the solution structure of the isolated F1 TnC C-terminal domain in the absence of calcium and have compared it together with its dynamical properties with those of the calcium-loaded form. The domain is folded also in the absence of calcium and is in a closed conformation. Binding of a single calcium is sufficient to induce a modest but clear closed-to-open conformational transition and releases the conformational entropy observed in the calcium-free form. These results provide the first example of a TnC domain in which the presence of only one calcium ion is sufficient to induce a closed-to-open transition and clarify the role of calcium in stretch activation.


===Solution Structure of the Apo C terminal domain of Lethocerus troponin C isoform F1===
Solution structure of the Apo C-terminal domain of the Lethocerus F1 troponin C isoform.,De Nicola GF, Martin S, Bullard B, Pastore A Biochemistry. 2010 Mar 2;49(8):1719-26. PMID:20104876<ref>PMID:20104876</ref>


{{ABSTRACT_PUBMED_20104876}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2k2a" style="background-color:#fffaf0;"></div>
[[2k2a]] is a 1 chain structure of [[Troponin]] with sequence from [http://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2A OCA].


==See Also==
==See Also==
*[[Troponin|Troponin]]
*[[Troponin 3D structures|Troponin 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020104876</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lethocerus indicus]]
[[Category: Lethocerus indicus]]
[[Category: Bullard, B.]]
[[Category: Bullard B]]
[[Category: Kelly, G.]]
[[Category: De Nicola GF]]
[[Category: McCormick, J.]]
[[Category: Kelly G]]
[[Category: Nicola, G F.De.]]
[[Category: McCormick J]]
[[Category: Calcium binding protein]]
[[Category: Contractile protein]]
Retrieved from "https://proteopedia.org/2k2a"