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[[Image:3ie2.png|left|200px]]


{{STRUCTURE_3ie2|  PDB=3ie2  |  SCENE=  }}
==Crystal Structure of H400V mutant TTHA0252 from Thermus thermophilus HB8==
 
<StructureSection load='3ie2' size='340' side='right'caption='[[3ie2]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
===Crystal Structure of H400V mutant TTHA0252 from Thermus thermophilus HB8===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3ie2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2yvd 2yvd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IE2 FirstGlance]. <br>
 
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
==About this Structure==
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
[[3ie2]] is a 4 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2yvd 2yvd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IE2 OCA].  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ie2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ie2 OCA], [https://pdbe.org/3ie2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ie2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ie2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ie2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNSE_THET8 RNSE_THET8] Has endoribonuclease activity towards 23S and 16S rRNA (in vitro).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/3ie2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ie2 ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Ribonuclease|Ribonuclease]]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
[[Category: Thermus thermophilus]]
__TOC__
[[Category: Ishikawa, H.]]
</StructureSection>
[[Category: Kuramitsu, S.]]
[[Category: Large Structures]]
[[Category: Masui, R.]]
[[Category: Thermus thermophilus HB8]]
[[Category: Nakagawa, N.]]
[[Category: Ishikawa H]]
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Kuramitsu S]]
[[Category: Yokoyama, S.]]
[[Category: Masui R]]
[[Category: Endonuclease]]
[[Category: Nakagawa N]]
[[Category: Hydrolase]]
[[Category: Yokoyama S]]
[[Category: Metal-binding]]
[[Category: Metallo beta lactamase fold]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Nuclease]]
[[Category: Riken structural genomics/proteomics initiative]]
[[Category: Rna-binding]]
[[Category: Rrna processing]]
[[Category: Rsgi]]
[[Category: Structural genomic]]

Latest revision as of 15:57, 1 November 2023

Crystal Structure of H400V mutant TTHA0252 from Thermus thermophilus HB8

3ie2, resolution 2.80Å

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