2cbf: Difference between revisions

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[[Image:2cbf.gif|left|200px]]<br />
<applet load="2cbf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2cbf, resolution 3.1&Aring;" />
'''THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF'''<br />


==Overview==
==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==
Biosynthesis of the corrin ring of vitamin B12 requires the action of six, S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely, related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been, determined to a resolution of 2.4 A. CbiF contains two alphabeta domains, forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The, location of AdoHcy and a number of conserved residues, helps define the, precorrin binding site. A second crystal form determined at 3.1 A, resolution highlights the flexibility of two loops around this site. CbiF, employs a unique mode of AdoHcy binding and represents a new class of, transmethylase.
<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
2CBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]] with SAH as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133]]. Structure known Active Sites: FLP and SAH. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665173 9665173]
</table>
[[Category: Bacillus megaterium]]
== Function ==
[[Category: Precorrin-4 C(11)-methyltransferase]]
[https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Raux, E.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Schubert, H.L.]]
Check<jmol>
[[Category: Warren, M.J.]]
  <jmolCheckbox>
[[Category: Wilson, K.S.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/2cbf_consurf.spt"</scriptWhenChecked>
[[Category: Woodcock, S.C.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: SAH]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: cobalamin biosynthesis]]
  </jmolCheckbox>
[[Category: methylase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf].
[[Category: precorrin-4 methyltransferase]]
<div style="clear:both"></div>
 
== References ==
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:00:33 2007''
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Priestia megaterium]]
[[Category: Raux E]]
[[Category: Schubert HL]]
[[Category: Warren MJ]]
[[Category: Wilson KS]]
[[Category: Woodcock SC]]
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