3q8m: Difference between revisions
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==Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in complex with substrate 5'-flap DNA and K+== | |||
<StructureSection load='3q8m' size='340' side='right'caption='[[3q8m]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3q8m]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q8M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
== | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q8m OCA], [https://pdbe.org/3q8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q8m RCSB], [https://www.ebi.ac.uk/pdbsum/3q8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q8m ProSAT]</span></td></tr> | ||
[[3q8m]] is a 8 chain structure | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/FEN1_HUMAN FEN1_HUMAN] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.<ref>PMID:7961795</ref> <ref>PMID:8621570</ref> <ref>PMID:10744741</ref> <ref>PMID:11986308</ref> <ref>PMID:18443037</ref> <ref>PMID:20729856</ref> | |||
==See Also== | ==See Also== | ||
*[[Endonuclease|Endonuclease]] | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Arvai | [[Category: Large Structures]] | ||
[[Category: Chapados | [[Category: Arvai A]] | ||
[[Category: Classen | [[Category: Chapados BR]] | ||
[[Category: Cooper | [[Category: Classen S]] | ||
[[Category: Finger | [[Category: Cooper PK]] | ||
[[Category: Grasby | [[Category: Finger DL]] | ||
[[Category: Guenther | [[Category: Grasby JA]] | ||
[[Category: Sarker | [[Category: Guenther G]] | ||
[[Category: Shen | [[Category: Sarker AH]] | ||
[[Category: Tainer | [[Category: Shen B]] | ||
[[Category: Thompson | [[Category: Tainer JA]] | ||
[[Category: Tomlinson | [[Category: Thompson P]] | ||
[[Category: Tsutakawa | [[Category: Tomlinson CG]] | ||
[[Category: Tsutakawa SE]] | |||
Latest revision as of 11:37, 14 March 2024
Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in complex with substrate 5'-flap DNA and K+
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