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| [[Image:1g6u.jpg|left|200px]]<br /><applet load="1g6u" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1g6u, resolution 1.48Å" />
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| '''CRYSTAL STRUCTURE OF A DOMAIN SWAPPED DIMER'''<br />
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| ==Overview== | | ==CRYSTAL STRUCTURE OF A DOMAIN SWAPPED DIMER== |
| Three-dimensional (3D) domain-swapped proteins are intermolecularly folded analogs of monomeric proteins; both are stabilized by the identical interactions, but the individual domains interact intramolecularly in monomeric proteins, whereas they form intermolecular interactions in 3D domain-swapped structures. The structures and conditions of formation of several domain-swapped dimers and trimers are known, but the formation of higher order 3D domain-swapped oligomers has been less thoroughly studied. Here we contrast the structural consequences of domain swapping from two designed three-helix bundles: one with an up-down-up topology, and the other with an up-down-down topology. The up-down-up topology gives rise to a domain-swapped dimer whose structure has been determined to 1.5 A resolution by x-ray crystallography. In contrast, the domain-swapped protein with an up-down-down topology forms fibrils as shown by electron microscopy and dynamic light scattering. This demonstrates that design principles can predict the oligomeric state of 3D domain-swapped molecules, which should aid in the design of domain-swapped proteins and biomaterials.
| | <StructureSection load='1g6u' size='340' side='right'caption='[[1g6u]], [[Resolution|resolution]] 1.48Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1g6u]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6U FirstGlance]. <br> |
| 1G6U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TFA:'>TFA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6U OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6u OCA], [https://pdbe.org/1g6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6u RCSB], [https://www.ebi.ac.uk/pdbsum/1g6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6u ProSAT]</span></td></tr> |
| Design of three-dimensional domain-swapped dimers and fibrous oligomers., Ogihara NL, Ghirlanda G, Bryson JW, Gingery M, DeGrado WF, Eisenberg D, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1404-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11171963 11171963]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Bryson, J W.]] | | </StructureSection> |
| [[Category: DeGrado, W F.]] | | [[Category: Large Structures]] |
| [[Category: Eisenberg, D.]] | | [[Category: Bryson JW]] |
| [[Category: Ghirlanda, G.]] | | [[Category: DeGrado WF]] |
| [[Category: Gingery, M.]] | | [[Category: Eisenberg D]] |
| [[Category: Ogihara, N L.]] | | [[Category: Ghirlanda G]] |
| [[Category: SO4]]
| | [[Category: Gingery M]] |
| [[Category: TFA]]
| | [[Category: Ogihara NL]] |
| [[Category: designed three helix bundle]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:44 2008''
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