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[[Image:1gam.jpg|left|200px]]<br /><applet load="1gam" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1gam, resolution 2.6&Aring;" />
'''GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN'''<br />


==Overview==
==GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN==
We use protein engineering and crystallography to simulate aspects of the early evolution of beta gamma-crystallins by observing how a single domain oligomerizes in response to changes in a sequence extension. The crystal structure of the C-terminal domain of gamma beta-crystallin with its four-residue C-terminal extension shows that the domain does not form a symmetric homodimer analogous to the two-domain pairing in beta gamma-crystallins. Instead the C-terminal extension now forms heterologous interactions with other domains leading to the solvent exposure of the natural hydrophobic interface with a consequent loss in protein solubility. However, this domain truncated by just the C-terminal tyrosine forms a symmetric homodimer of domains in the crystal lattice.
<StructureSection load='1gam' size='340' side='right'caption='[[1gam]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GAM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gam OCA], [https://pdbe.org/1gam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gam RCSB], [https://www.ebi.ac.uk/pdbsum/1gam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gam ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRGB_BOVIN CRGB_BOVIN] Crystallins are the dominant structural components of the vertebrate eye lens.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/1gam_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gam ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GAM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GAM OCA].
*[[Crystallin 3D structures|Crystallin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins., Norledge BV, Mayr EM, Glockshuber R, Bateman OA, Slingsby C, Jaenicke R, Driessen HP, Nat Struct Biol. 1996 Mar;3(3):267-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8605629 8605629]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bateman, O A.]]
[[Category: Bateman OA]]
[[Category: Driessen, H P.C.]]
[[Category: Driessen HPC]]
[[Category: Glockshuber, R.]]
[[Category: Glockshuber R]]
[[Category: Jaenicke, R.]]
[[Category: Jaenicke R]]
[[Category: Mayr, E M.]]
[[Category: Mayr E-M]]
[[Category: Norledge, B V.]]
[[Category: Norledge BV]]
[[Category: Slingsby, C.]]
[[Category: Slingsby C]]
[[Category: eye lens protein]]
[[Category: gamma crystallin b]]
[[Category: multigene family]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:48:03 2008''

Latest revision as of 07:24, 7 February 2024

GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN

1gam, resolution 2.60Å

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