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[[Image:3kxd.png|left|200px]]


{{STRUCTURE_3kxd| PDB=3kxd | SCENE= }}
==Crystal structure of the mthk rck in complex with cadmium==
<StructureSection load='3kxd' size='340' side='right'caption='[[3kxd]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3kxd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KXD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxd OCA], [https://pdbe.org/3kxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kxd RCSB], [https://www.ebi.ac.uk/pdbsum/3kxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kxd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kxd ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RCK is a cytoplasmic regulatory domain of calcium-gated potassium channels. Binding of Ca(2+) by RCK leads to channel activation through a series of yet unknown conformational changes. Structures of the K(+) channel, MthK, and its cytoplasmic RCK domain revealed two binding sites for Ca(2+) per dimer. We determined the crystal structure of RCK in complex with Cd(2+) at 2.2A resolution. Cd(2+) activates MthK more efficiently, and binds at the same binding sites for Ca(2+) but with reduced coordination number. Two additional binding sites for Cd(2+) are found per dimer; one on the main Rossman-fold lobe, and the other on the small lobe of RCK. Using patch-clamp experiments, we demonstrate that Cd(2+) binding to these novel sites enhances activation by Cd(2+) and not by Ca(2+). The structure reveals a large negatively charged surface patch in the proximity of the Ca(2+)/Cd(2+) binding sites, charge neutralization of which appears to promote the channel open state.


===Crystal structure of the mthk rck in complex with cadmium===
Structure of the MthK RCK in complex with cadmium.,Dvir H, Valera E, Choe S J Struct Biol. 2010 Aug;171(2):231-7. Epub 2010 Apr 4. PMID:20371380<ref>PMID:20371380</ref>


{{ABSTRACT_PUBMED_20371380}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3kxd" style="background-color:#fffaf0;"></div>
[[3kxd]] is a 2 chain structure of [[Potassium Channel]] with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._delta_h Methanothermobacter thermautotrophicus str. delta h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA].


==See Also==
==See Also==
*[[Potassium Channel|Potassium Channel]]
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020371380</ref><references group="xtra"/>
__TOC__
[[Category: Methanothermobacter thermautotrophicus str. delta h]]
</StructureSection>
[[Category: Choe, S.]]
[[Category: Large Structures]]
[[Category: Dvir, H.]]
[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
[[Category: Alternative initiation]]
[[Category: Choe S]]
[[Category: Cadmium]]
[[Category: Dvir H]]
[[Category: Cell membrane]]
[[Category: Ion transport]]
[[Category: Ionic channel]]
[[Category: Membrane]]
[[Category: Metal-binding]]
[[Category: Mthk]]
[[Category: Potassium]]
[[Category: Potassium channel]]
[[Category: Potassium transport]]
[[Category: Rck]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/3kxd"