G13secL03Tpc3: Difference between revisions
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==Structure== | ==Structure== | ||
<Structure load='1RJL' size=' | <Structure load='1RJL' size='350' frame='true' align='right' scene='G13secL03Tpc3/Final_ospb/2'/> | ||
<Structure load='1RJL' size=' | <Structure load='1RJL' size='350' frame='true' align='right' scene='G13secL03Tpc3/Final_bsheet/2'/> | ||
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A more significant <scene name='G13secL03Tpc3/Final_bsheet/3'>structural change</scene> that occurs during the bactericidal Fab complex is the removal of OspB's first four beta sheets. Surrounding sheets shift and overlap to replace the missing sheets. The removal of these sheets can affect the outer surface protein and make the bacteria more susceptible to lysis. | A more significant <scene name='G13secL03Tpc3/Final_bsheet/3'>structural change</scene> that occurs during the bactericidal Fab complex is the removal of OspB's first four beta sheets. Surrounding sheets shift and overlap to replace the missing sheets. The removal of these sheets can affect the outer surface protein and make the bacteria more susceptible to lysis. | ||
=== Destruction=== | === Destruction=== | ||
The bacteria lyses indirectly. The proteolytic activity of the antibody on the bacteria is presumably similar to a membrane attack complex (MAC). The complement system activates the classical pathway, activating effector proteins which insert themselves in the outer membrane of the bacteria and disrupting it and causing death to the cell. <ref name=Article3> PMID:15864264 </ref> | The bacteria lyses indirectly. The proteolytic activity of the antibody on the bacteria is presumably similar to a [http://en.wikipedia.org/wiki/Complement_membrane_attack_complex membrane attack complex] (MAC). The complement system activates the classical pathway, activating effector proteins which insert themselves in the outer membrane of the bacteria and disrupting it and causing death to the cell. <ref name=Article3> PMID:15864264 </ref> | ||
[[Image:Mac attack.PNG|thumb|400px|Mechanism of destruction by MAC on the bacteria, ''Borrelia burgdorferi'']] | [[Image:Mac attack.PNG|thumb|400px|Mechanism of destruction by MAC on the bacteria, ''Borrelia burgdorferi'']] | ||
==Application== | ==Application== | ||
Under pressure in the presence of antibodies, OspB is capable of existing in various forms. Mutations occur in the residue of Lysine 253, the epitope of H6831, and may prevent Borrelia from being recognized and lysed by bactericidal Fabs such as H6381. <ref name=Article4> PMID: 15128807 </ref> The study of this structure of Osp B is important to combat future mechanisms of curing Lyme disease. Vaccines created to specifically fight OspB have not yet been successful, due to the increased variability of the | Under pressure in the presence of antibodies, OspB is capable of existing in various forms. Mutations occur in the residue of Lysine 253, the epitope of H6831, and may prevent ''Borrelia'' from being recognized and lysed by bactericidal Fabs such as H6381. <ref name=Article4> PMID: 15128807 </ref> The study of this structure of Osp B is important to combat future mechanisms of curing Lyme disease. Vaccines created to specifically fight OspB have not yet been successful, due to the increased variability of the epitope. Vaccine are not yet able to counteract the varied strains of ''Borrelia burgdorferi.'' However, the ability of H6381 and CB2 to make the bacteria subject to increased proteolytic activity is a possibility for future research, as it is still currently misunderstood. | ||
References: {{reflist}} | References: {{reflist}} | ||
