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[[Image:1j7d.jpg|left|200px]]<br /><applet load="1j7d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1j7d, resolution 1.85&Aring;" />
'''Crystal Structure of hMms2-hUbc13'''<br />


==Overview==
==Crystal Structure of hMms2-hUbc13==
The ubiquitin conjugating enzyme complex Mms2-Ubc13 plays a key role in post-replicative DNA repair in yeast and the NF-kappaB signal transduction pathway in humans. This complex assembles novel polyubiquitin chains onto yet uncharacterized protein targets. Here we report the crystal structure of a complex between hMms2 (Uev1) and hUbc13 at 1.85 A resolution and a structure of free hMms2 at 1.9 A resolution. These structures reveal that the hMms2 monomer undergoes a localized conformational change upon interaction with hUbc13. The nature of the interface provides a physical basis for the preference of Mms2 for Ubc13 as a partner over a variety of other structurally similar ubiquitin-conjugating enzymes. The structure of the hMms2-hUbc13 complex provides the conceptual foundation for understanding the mechanism of Lys 63 multiubiquitin chain assembly and for its interactions with the RING finger proteins Rad5 and Traf6.
<StructureSection load='1j7d' size='340' side='right'caption='[[1j7d]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1j7d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J7D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j7d OCA], [https://pdbe.org/1j7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j7d RCSB], [https://www.ebi.ac.uk/pdbsum/1j7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j7d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UB2V2_HUMAN UB2V2_HUMAN] Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.<ref>PMID:9705497</ref> <ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:20061386</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/1j7d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j7d ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1J7D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7D OCA].
*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
 
== References ==
==Reference==
<references/>
Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13., Moraes TF, Edwards RA, McKenna S, Pastushok L, Xiao W, Glover JN, Ellison MJ, Nat Struct Biol. 2001 Aug;8(8):669-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11473255 11473255]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Ubiquitin--protein ligase]]
[[Category: Edwards RA]]
[[Category: Edwards, R A.]]
[[Category: Ellison MJ]]
[[Category: Ellison, M J.]]
[[Category: Glover JNM]]
[[Category: Glover, J N.M.]]
[[Category: McKenna S]]
[[Category: McKenna, S.]]
[[Category: Moraes TF]]
[[Category: Moraes, T F.]]
[[Category: Pashushok L]]
[[Category: Pashushok, L.]]
[[Category: Xiao W]]
[[Category: Xiao, W.]]
[[Category: dna repair]]
[[Category: nfkb]]
[[Category: traf6]]
[[Category: ubc]]
[[Category: ubiquitin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:30 2008''

Latest revision as of 07:37, 7 February 2024

Crystal Structure of hMms2-hUbc13

1j7d, resolution 1.85Å

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