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[[Image:1jac.gif|left|200px]]<br /><applet load="1jac" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jac, resolution 2.43&Aring;" />
'''A NOVEL MODE OF CARBOHYDRATE RECOGNITION IN JACALIN, A MORACEAE PLANT LECTIN WITH A BETA-PRISM'''<br />


==Overview==
==A NOVEL MODE OF CARBOHYDRATE RECOGNITION IN JACALIN, A MORACEAE PLANT LECTIN WITH A BETA-PRISM==
Jacalin, a tetrameric two-chain lectin (66,000 Mr) from jackfruit seeds, is highly specific for the tumour associated T-antigenic disaccharide. The crystal structure of jacalin with methyl-alpha-D-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. The lectin exhibits a novel carbohydrate-binding site involving the N terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis, the first known instance where such a modification has been used to confer carbohydrate specificity. This new lectin fold may be characteristic of the Moraceae plant family. The structure provides an explanation for the relative affinities of the lectin for galactose derivatives and provides insights into the structural basis of its T-antigen specificity.
<StructureSection load='1jac' size='340' side='right'caption='[[1jac]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jac]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_heterophyllus Artocarpus heterophyllus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jac OCA], [https://pdbe.org/1jac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jac RCSB], [https://www.ebi.ac.uk/pdbsum/1jac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jac ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jac_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jac ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JAC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_heterophyllus Artocarpus heterophyllus] with <scene name='pdbligand=AMG:'>AMG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAC OCA].
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold., Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M, Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8673603 8673603]
[[Category: Artocarpus heterophyllus]]
[[Category: Artocarpus heterophyllus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Banerjee, R.]]
[[Category: Banerjee R]]
[[Category: Sankaranarayanan, R.]]
[[Category: Sankaranarayanan R]]
[[Category: Sekar, K.]]
[[Category: Sekar K]]
[[Category: Sharma, V.]]
[[Category: Sharma V]]
[[Category: Surolia, A.]]
[[Category: Surolia A]]
[[Category: Vijayan, M.]]
[[Category: Vijayan M]]
[[Category: AMG]]
[[Category: beta-prism fold]]
[[Category: carbohydrate specificity]]
[[Category: glycoprotein]]
[[Category: lectin]]
[[Category: non-legume]]
 
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Retrieved from "https://proteopedia.org/1jac"