1jif: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(12 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1jif.gif|left|200px]]<br /><applet load="1jif" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jif, resolution 1.6&Aring;" />
'''Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin'''<br />


==Overview==
==Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin==
Bleomycin (Bm) in the culture broth of Streptomyces verticillus is complexed with Cu(2+) (Cu(II)). In the present study, we determined the x-ray crystal structures of the Cu(II)-bound and the metal-free types of Bm at a high resolution of 1.6 and 1.8 A, respectively, which are complexed with a Bm resistance determinant from Bm-producing S. verticillus, designated BLMA. In the current model of Cu(II).Bm complexed with BLMA, two Cu(II).Bm molecules bind to the BLMA dimer. The electron density map shows that the copper ion is clearly defined in the metal-binding domain of the Bm molecule. The metal ion is penta-coordinated by a tetragonal monopyramidal cage of nitrogens and binds to the primary amine of the beta-aminoalanine moiety of Bm. The binding experiment between Bm and BLMA showed that each of the two Bm-binding pockets has a different dissociation constant (K(d)(1) and K(d)(2)). The K(d)(1) value of 630 nm for the first Bm binding is larger than the K(d)(2) value of 120 nm, indicating that the first Bm binding gives rise to a cooperative binding of the second Bm to the other pocket.
<StructureSection load='1jif' size='340' side='right'caption='[[1jif]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1jif]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JIF FirstGlance]. <br>
1JIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=BLM:'>BLM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIF OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jif OCA], [https://pdbe.org/1jif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jif RCSB], [https://www.ebi.ac.uk/pdbsum/1jif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jif ProSAT]</span></td></tr>
The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus., Sugiyama M, Kumagai T, Hayashida M, Maruyama M, Matoba Y, J Biol Chem. 2002 Jan 18;277(3):2311-20. Epub 2001 Nov 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11706014 11706014]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/Q53793_9ACTN Q53793_9ACTN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1jif_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jif ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces verticillus]]
[[Category: Streptomyces verticillus]]
[[Category: Hayashida, M.]]
[[Category: Hayashida M]]
[[Category: Kumagai, T.]]
[[Category: Kumagai T]]
[[Category: Maruyama, M.]]
[[Category: Maruyama M]]
[[Category: Matoba, Y.]]
[[Category: Matoba Y]]
[[Category: Sugiyama, M.]]
[[Category: Sugiyama M]]
[[Category: BLM]]
[[Category: CL]]
[[Category: CU]]
[[Category: protein-ligand complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:00 2008''

Latest revision as of 13:24, 13 March 2024

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin

1jif, resolution 1.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1jif&oldid=4087317"