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[[Image:3uv3.jpg|left|200px]]


{{STRUCTURE_3uv3| PDB=3uv3 | SCENE= }}
==Ec_IspH in complex with but-2-ynyl diphosphate (1086)==
<StructureSection load='3uv3' size='340' side='right'caption='[[3uv3]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3uv3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UV3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0CM:BUT-2-YN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0CM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uv3 OCA], [https://pdbe.org/3uv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uv3 RCSB], [https://www.ebi.ac.uk/pdbsum/3uv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uv3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The final step of the methylerythritol phosphate isoprenoid biosynthesis pathway is catalysed by the iron-sulphur enzyme IspH, producing the universal precursors of terpenes: isopentenyl diphosphate and dimethylallyl diphosphate. Here we report an unforeseen reaction discovered during the investigation of the interaction of IspH with acetylene inhibitors by X-ray crystallography, Mossbauer, and nuclear magnetic resonance spectroscopy. In addition to its role as a 2H(+)/2e(-) reductase, IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. The reactions only occur with the oxidised protein and proceed via eta(1)-O-enolate intermediates. One of these is characterized crystallographically and contains a C4 ligand oxygen bound to the unique, fourth iron in the 4Fe-4S cluster: this intermediate subsequently hydrolyzes to produce an aldehyde product. This unexpected side to IspH reactivity is of interest in the context of the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives targeting IspH.


===Ec_IspH in complex with but-2-ynyl diphosphate (1086)===
Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.,Span I, Wang K, Wang W, Zhang Y, Bacher A, Eisenreich W, Li K, Schulz C, Oldfield E, Groll M Nat Commun. 2012 Sep 4;3:1042. doi: 10.1038/ncomms2052. PMID:22948824<ref>PMID:22948824</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3uv3" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[3uv3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UV3 OCA].
*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures|4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures]]
[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
== References ==
[[Category: Escherichia coli]]
<references/>
[[Category: Bacher, A.]]
__TOC__
[[Category: Eisenreich, W.]]
</StructureSection>
[[Category: Groll, M.]]
[[Category: Escherichia coli K-12]]
[[Category: Oldfield, E.]]
[[Category: Large Structures]]
[[Category: Schulz, C.]]
[[Category: Bacher A]]
[[Category: Span, I.]]
[[Category: Eisenreich W]]
[[Category: Wang, K.]]
[[Category: Groll M]]
[[Category: Wang, W.]]
[[Category: Oldfield E]]
[[Category: Zhang, Y.]]
[[Category: Schulz C]]
[[Category: Iron-sulfur protein]]
[[Category: Span I]]
[[Category: Isoprenoid biosynthesis]]
[[Category: Wang K]]
[[Category: Isph]]
[[Category: Wang W]]
[[Category: Lytb]]
[[Category: Zhang Y]]
[[Category: Non-mevalonate pathway]]
[[Category: Oxidoreductase]]
Retrieved from "https://proteopedia.org/3uv3"