Enolase multiple sequence alignment: Difference between revisions

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This page is a supplement to [[Introduction to Evolutionary Conservation]]. The portion of the multiple sequence alignment (MSA) below with <span style="background:pink;">&nbsp;a pink background&nbsp;</span> is enlarged on that page.
----
Multiple Sequence Alignment (MSA) for enolase, an enzyme in glycolysis.
Multiple Sequence Alignment (MSA) for enolase, an enzyme in glycolysis.


Key:  IDENTICAL(*)   SIMILAR(.)   different  
Key:  '''IDENTICAL'''(*) &nbsp;  SIMILAR(.) &nbsp; different  
 
Consensus Percentage is set to 100%.  
Consensus Percentage is set to 100%.  


 
----
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mso-bidi-font-family:"Courier New"'>&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME|
mso-bidi-font-family:"Courier New"'>&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME|
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EVytetgafg rAlv<b>PSGAST</b> 43<br>
EVytetgafg rAlv<b>PSGAST</b> 43<br>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;---------a VskVyA<b>R</b>sVy
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;---------a VskVyA<b>R</b>sVy
<b>DSRGNPTVE</b>v ELtt-ekgvf rSiv<b>PSGAST</b> 40<o:p></o:p></span></p>
<b>DSRGNPTVE</b>v ELtt-ekgvf rSiv<b>PSGAST</b> 40</span></p>


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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>G</b>vh<b>EA</b>L<b>E</b>m<b>RD</b>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>G</b>vh<b>EA</b>L<b>E</b>m<b>RD</b>
gD<b>K</b>skWm<b>GK</b>G <b>V</b>lh<b>AV</b>knV<b>N</b>d vIapafVkan idvsd<b>Q</b>kaV<b>D</b>
gD<b>K</b>skWm<b>GK</b>G <b>V</b>lh<b>AV</b>knV<b>N</b>d vIapafVkan idvsd<b>Q</b>kaV<b>D</b>
90<o:p></o:p></span></p>
90</span></p>


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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;dfl<b>I</b>sl<b>DGT</b>a
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;dfl<b>I</b>sl<b>DGT</b>a
<b>NK</b>SKl<b>GANAI</b> <b>L</b>G<b>VS</b>l<b>A</b>asR<b>A</b> A<b>A</b>aeknv<b>PLY</b>
<b>NK</b>SKl<b>GANAI</b> <b>L</b>G<b>VS</b>l<b>A</b>asR<b>A</b> A<b>A</b>aeknv<b>PLY</b>
khLAdlsksk 140<o:p></o:p></span></p>
khLAdlsksk 140</span></p>


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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;tspyvl<b>PVP</b>f
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;tspyvl<b>PVP</b>f
l<b>N</b>VL<b>NGG</b>s<b>HA</b> ggaLal<b>QEFM</b> <b>I</b>a<b>P</b>t<b>GA</b>ktfa
l<b>N</b>VL<b>NGG</b>s<b>HA</b> ggaLal<b>QEFM</b> <b>I</b>a<b>P</b>t<b>GA</b>ktfa
<b>EA</b>lRi<b>G</b>SeVY 190<o:p></o:p></span></p>
<b>EA</b>lRi<b>G</b>SeVY 190</span></p>


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style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span>.<span style="mso-spacerun:
style="mso-spacerun: yes">&nbsp;&nbsp;&nbsp; </span>.<span style="mso-spacerun:
yes">&nbsp; </span>**** * * **<span style="mso-spacerun:
yes">&nbsp; </span>**** * * **<span style="mso-spacerun:
yes">&nbsp;&nbsp;&nbsp;&nbsp; </span>** . *. ..<o:p></o:p></span></p>
yes">&nbsp;&nbsp;&nbsp;&nbsp; </span>** . *. ..</span></p>


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ne<b>E</b>A<b>L</b>qtIve AIek<b>AG</b>fkpg 234<br>
ne<b>E</b>A<b>L</b>qtIve AIek<b>AG</b>fkpg 234<br>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>H</b>n<b>L</b>ksLtkkR
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>H</b>n<b>L</b>ksLtkkR
ygasagn<b>VGD</b> <b>EGG</b>v<b>AP</b>nIqt ae<b>E</b>A<b>L</b>dlIvd AIka<b>AG</b>hd&#8212;238<o:p></o:p></span></p>
ygasagn<b>VGD</b> <b>EGG</b>v<b>AP</b>nIqt ae<b>E</b>A<b>L</b>dlIvd AIka<b>AG</b>---- 238</span></p>


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yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</span><span style="mso-spacerun:
</span><span style="mso-spacerun:
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>*
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>**<span style="mso-spacerun: yes">&nbsp; </span>.<span style="mso-spacerun:
*<span style="mso-spacerun: yes">&nbsp; </span>.<span style="mso-spacerun:
yes">&nbsp;&nbsp; </span>.<span style="mso-spacerun:
yes">&nbsp;&nbsp; </span>.<span style="mso-spacerun:
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>*** *** ** .<span
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>*** *** ** .<span
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<span style="mso-spacerun:
<span style="mso-spacerun:
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</span>01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)~<br>
</span>
&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME| Drosophila melanogaster &nbsp;gkIeiGm<b>D</b>v<b>A</b>
 
A<b>SEF</b>Yk--d<b>G</b> q<b>Y</b>dLdfknek sdksqwlpad klanlykefi 285<br>
<br>
&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME| Drosophila melanogaster <span style="background:pink;">&nbsp;gkIeiGm<b>D</b>v<b>A</b>
A<b>SEF</b>Yk--d<b>G</b></span> q<b>Y</b>dLdfknek sdksqwlpad klanlykefi 285<br>
&nbsp;2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENOA_HUMAN|
&nbsp;2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENOA_HUMAN|
Homo sapiens &nbsp;dkVviGm<b>D</b>v<b>A</b> A<b>SEF</b>Fr--s<b>G</b> k<b>Y</b>dLdfksp-
Homo sapiens <span style="background:pink;">&nbsp;dkVviGm<b>D</b>v<b>A</b> A<b>SEF</b>Fr--s<b>G</b></span> k<b>Y</b>dLdfksp-
ddpsryispd qladlyksfi 283<br>
ddpsryispd qladlyksfi 283<br>
&nbsp;3&nbsp;&nbsp;&nbsp;&nbsp;ENO_METJA| Methanococcus janaschii &nbsp;deVvfAl<b>D</b>a<b>A</b>
&nbsp;3&nbsp;&nbsp;&nbsp;&nbsp;ENO_METJA| Methanococcus janaschii <span style="background:pink;">&nbsp;deVvfAl<b>D</b>a<b>A</b>
A<b>SEF</b>Yk--d<b>G</b> y<b>Y</b>yVegkk-- ------ltre elldyykalv 280<br>
A<b>SEF</b>Yk--d<b>G</b></span> y<b>Y</b>yVegkk-- ------ltre elldyykalv 280<br>
&nbsp;4&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENO_ECOLI|
&nbsp;4&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENO_ECOLI|
Escherichia coli &nbsp;kdItlAm<b>D</b>c<b>A</b> A<b>SEF</b>Yk--d<b>G</b> k<b>Y</b>vLagegn-
Escherichia coli <span style="background:pink;">&nbsp;kdItlAm<b>D</b>c<b>A</b> A<b>SEF</b>Yk--d<b>G</b></span> k<b>Y</b>vLagegn-
----kaftse efthfleelt 280<br>
----kaftse efthfleelt 280<br>
&nbsp;5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENO_BACSU|
&nbsp;5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ENO_BACSU|
Bacillus subtilis &nbsp;eeVklAm<b>D</b>a<b>A</b> S<b>SEF</b>Ynked<b>G</b> k<b>Y</b>hLsgeg--
Bacillus subtilis <span style="background:pink;">&nbsp;eeVklAm<b>D</b>a<b>A</b> S<b>SEF</b>Ynked<b>G</b></span> k<b>Y</b>hLsgeg--
----vvktsa emvdwyeelv 278<br>
----vvktsa emvdwyeelv 278<br>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;gkVkiGl<b>D</b>c<b>A</b>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae <span style="background:pink;">&nbsp;gkVkiGl<b>D</b>c<b>A</b>
S<b>SEF</b>Fk--d<b>G</b> k<b>Y</b>dLdfknpn sdkskwltgp qladlyhslm 286<o:p></o:p></span></p>
S<b>SEF</b>Fk--d<b>G</b></span> k<b>Y</b>dLdfknpn sdkskwltgp qladlyhslm 286</span></p>


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yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
yes">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</span>01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)01:37, 1 October 2012 (IST)~<br>
</span><br>
&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME| Drosophila melanogaster &nbsp;kdF<b>P</b>IV<b>SIED</b>
&nbsp;1&nbsp;&nbsp;&nbsp;&nbsp;ENO_DROME| Drosophila melanogaster &nbsp;kdF<b>P</b>IV<b>SIED</b>
pfdqdhWeAw snltgctd-- I<b>Q</b>I<b>V</b>G<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAV
pfdqdhWeAw snltgctd-- I<b>Q</b>I<b>V</b>G<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAV
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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;krY<b>P</b>IV<b>SIED</b>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;krY<b>P</b>IV<b>SIED</b>
pfaeddWeAw shffktag-- I<b>Q</b>I<b>V</b>A<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAI
pfaeddWeAw shffktag-- I<b>Q</b>I<b>V</b>A<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAI
334<o:p></o:p></span></p>
334</span></p>


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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;ekkaadaL<b>L</b>L
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;ekkaadaL<b>L</b>L
<b>K</b>v<b>NQIG</b>tLs<b>E</b> sik<b>A</b>aqdSfa a<b>G</b>WgvmV<b>SHR</b> <b>SGETED</b>tf<b>I</b>A
<b>K</b>v<b>NQIG</b>tLs<b>E</b> sik<b>A</b>aqdSfa a<b>G</b>WgvmV<b>SHR</b> <b>SGETED</b>tf<b>I</b>A
384<o:p></o:p></span></p>
384</span></p>


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&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>D</b>Lv<b>V</b>Glrt<b>GQ</b>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;<b>D</b>Lv<b>V</b>Glrt<b>GQ</b>
<b>IKTG</b>Apa<b>R</b>sE <b>R</b>l<b>AK</b>l<b>NQ</b>LL<b>R</b> <b>IE</b>eeLGdnav
<b>IKTG</b>Apa<b>R</b>sE <b>R</b>l<b>AK</b>l<b>NQ</b>LL<b>R</b> <b>IE</b>eeLGdnav
Fa<b>G</b>enfhhgd 434<o:p></o:p></span></p>
Fa<b>G</b>enfhhgd 434</span></p>


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style="mso-spacerun: yes">&nbsp; </span>* . * ** **..* **<span
style="mso-spacerun: yes">&nbsp; </span>* . * ** **..* **<span
style="mso-spacerun: yes">&nbsp; </span>..<span style="mso-spacerun:
style="mso-spacerun: yes">&nbsp; </span>..<span style="mso-spacerun:
yes">&nbsp;&nbsp;&nbsp;&nbsp; </span>. *<o:p></o:p></span></p>
yes">&nbsp;&nbsp;&nbsp;&nbsp; </span>. *</span></p>


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Bacillus subtilis &nbsp;nk 430<br>
Bacillus subtilis &nbsp;nk 430<br>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;kl 436<br>
&nbsp;6&nbsp;&nbsp;4ENL Yeast| Saccharomyces cerevisiae &nbsp;kl 436<br>
</span></p>
----
Summary of categories applied to a total of 436 residues in the PDB file sequence for a consensus level setting of 100%:


33% (142) IDENTICAL(*)  16% (68) SIMILAR(.)  52% (226) different


 
Definitions of SIMILAR groups of amino acids:<pre>
 
ILV   Ile Leu Val     hydrophobic, aliphatic / medium-sized side-chain
 
FWY   Phe Trp Tyr     hydrophobic, aromatic / large side-chain (Tyr polar)
Summary of categories applied to a total of 436 residues in the PDB file sequence for a consensus level setting of 100%:
KRH   Lys Arg His     + charge
33% (142) IDENTICAL(*)  16% (68) SIMILAR(.)  52% (226) different
DE   Asp Glu         - charge
Definitions of SIMILAR groups of amino acids:
GAS   Gly Ala Ser     small
ILV       Ile Leu Val         hydrophobic, aliphatic / medium-sized side-chain
P     Pro             helix breaking
FWY     Phe Trp Tyr         hydrophobic, aromatic / large side-chain (Tyr polar)
C     Cys             disulfide-forming
KRH     Lys Arg His         + charge
DE       Asp Glu               - charge
GAS     Gly Ala Ser           small
P           Pro                       helix breaking
C           Cys                       disulfide-forming
TNQM  Thr Asn Gln Met  polar (except Met)
TNQM  Thr Asn Gln Met  polar (except Met)
</pre>
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