2lr2: Difference between revisions
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==Designed IgG and lanthanide binding probe for solution NMR, MRI and luminescence microscopy== | |||
<StructureSection load='2lr2' size='340' side='right'caption='[[2lr2]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lr2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LR2 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lr2 OCA], [https://pdbe.org/2lr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lr2 RCSB], [https://www.ebi.ac.uk/pdbsum/2lr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lr2 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Paramagnetic lanthanide ions when bound to proteins offer great potential for structural investigations that utilize solution nuclear magnetic resonance spectroscopy, magnetic resonance imaging, or optical microscopy. However, many proteins do not have native metal ion binding sites and engineering a chimeric protein to bind an ion while retaining affinity for a protein of interest represents a significant challenge. Here we report the characterization of an immunoglobulin G-binding protein redesigned to include a lanthanide binding motif in place of a loop between two helices (Z-L2LBT). It was shown to bind Tb(3+) with 130 nM affinity. Ions such as Dy(3+) , Yb(3+) , and Ce(3+) produce paramagnetic effects on NMR spectra and the utility of these effects is illustrated by their use in determining a structural model of the metal-complexed Z-L2LBT protein and a preliminary characterization of the dynamic distribution of IgG Fc glycan positions. Furthermore, this designed protein is demonstrated to be a novel IgG-binding reagent for magnetic resonance imaging (Z-L2LBT:Gd(3+) complex) and luminescence microscopy (Z-L2LBT: Tb(3+) complex). | |||
Lanthanide binding and IgG affinity construct: Potential applications in solution NMR, MRI, and luminescence microscopy.,Barb AW, Ho TG, Flanagan-Steet H, Prestegard JH Protein Sci. 2012 Jul 31. doi: 10.1002/pro.2133. PMID:22851279<ref>PMID:22851279</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | <div class="pdbe-citations 2lr2" style="background-color:#fffaf0;"></div> | ||
[[ | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
[[Category: Barb | [[Category: Barb AW]] | ||
[[Category: Ho | [[Category: Ho TG]] | ||
[[Category: Prestegard | [[Category: Prestegard JH]] | ||
Latest revision as of 05:49, 15 May 2024
Designed IgG and lanthanide binding probe for solution NMR, MRI and luminescence microscopy
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