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[[Image:4akl.png|left|200px]]


{{STRUCTURE_4akl| PDB=4akl | SCENE= }}
==Structure of the Crimean-Congo Haemorrhagic Fever Virus Nucleocapsid Protein==
<StructureSection load='4akl' size='340' side='right'caption='[[4akl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4akl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_orthonairovirus Crimean-Congo hemorrhagic fever orthonairovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AKL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4akl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4akl OCA], [https://pdbe.org/4akl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4akl RCSB], [https://www.ebi.ac.uk/pdbsum/4akl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4akl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q70UR4_9VIRU Q70UR4_9VIRU]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging tick-borne virus of the Bunyaviridae family responsible for fatal human disease for which preventative or therapeutic measures do not exist. We solved the crystal structure of the Baghdad-12 strain CCHFV nucleocapsid protein (N), a potential therapeutic target, at a resolution of 2.1 A. N comprises a large globular domain composed of both N- and C-terminal sequences, likely involved in RNA binding, and a protruding 'arm' domain with a conserved DEVD caspase-3 cleavage site at its apex. Alignment of our structure with that from the recently reported N from strain YL04057 shows close correspondence of all folds, but significant transposition of the arm through a rotation of 180 degrees and translation of 40 A. These observations suggest structural flexibility that may provide the basis for switching between alternative N protein conformations during important functions such as RNA binding or oligomerization. Our structure reveals surfaces likely involved in RNA binding and oligomerization, and functionally-critical residues within these domains were identified using a mini-genome system able to recapitulate CCHFV-specific RNA synthesis in cells. Caspase-3 cleaves the polypeptide chain at the exposed DEVD motif, however the cleaved N protein remains as an intact unit, likely due to the intimate association of N- and C-terminal fragments in the globular domain. Structural alignment with existing N proteins reveals that the closest CCHFV relative is not another bunyavirus, but instead the arenavirus Lassa virus, suggesting that current segmented negative strand RNA virus taxonomy may need revision.


===Structure of the Crimean-Congo Haemorrhagic Fever Virus Nucleocapsid Protein===
Structure, function and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein.,Carter SD, Surtees R, Walter CT, Ariza A, Bergeron E, Nichol ST, Hiscox JA, Edwards TA, Barr JN J Virol. 2012 Aug 8. PMID:22875964<ref>PMID:22875964</ref>


{{ABSTRACT_PUBMED_22875964}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 4akl" style="background-color:#fffaf0;"></div>
[[4akl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crimean-congo_hemorrhagic_fever_virus Crimean-congo hemorrhagic fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AKL OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:022875964</ref><references group="xtra"/>
</StructureSection>
[[Category: Crimean-congo hemorrhagic fever virus]]
[[Category: Crimean-Congo hemorrhagic fever orthonairovirus]]
[[Category: Albarino, C G.]]
[[Category: Large Structures]]
[[Category: Ariza, A.]]
[[Category: Albarino CG]]
[[Category: Barr, J N.]]
[[Category: Ariza A]]
[[Category: Bergeron, E.]]
[[Category: Barr JN]]
[[Category: Carter, S D.]]
[[Category: Bergeron E]]
[[Category: Edwards, T A.]]
[[Category: Carter SD]]
[[Category: Hiscox, J A.]]
[[Category: Edwards TA]]
[[Category: Nichol, S T.]]
[[Category: Hiscox JA]]
[[Category: Surtees, R.]]
[[Category: Nichol ST]]
[[Category: Walter, C T.]]
[[Category: Surtees R]]
[[Category: Bunyaviridae]]
[[Category: Walter CT]]
[[Category: Caspase-3]]
[[Category: Cchfv]]
[[Category: Viral protein]]
Retrieved from "https://proteopedia.org/4akl"