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[[Image:1kke.gif|left|200px]]<br /><applet load="1kke" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1kke, resolution 2.6&Aring;" />
'''Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer'''<br />


==Overview==
==Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer==
Reovirus attaches to cellular receptors with the sigma1 protein, a fiber-like molecule protruding from the 12 vertices of the icosahedral virion. The crystal structure of a receptor-binding fragment of sigma1 reveals an elongated trimer with two domains: a compact head with a new beta-barrel fold and a fibrous tail containing a triple beta-spiral. Numerous structural and functional similarities between reovirus sigma1 and the adenovirus fiber suggest an evolutionary link in the receptor-binding strategies of these two viruses. A prominent loop in the sigma1 head contains a cluster of residues that are conserved among reovirus serotypes and are likely to form a binding site for junction adhesion molecule, an integral tight junction protein that serves as a reovirus receptor. The fibrous tail is mainly responsible for sigma1 trimer formation, and it contains a highly flexible region that allows for significant movement between the base of the tail and the head. The architecture of the trimer interface and the observed flexibility indicate that sigma1 is a metastable structure poised to undergo conformational changes upon viral attachment and cell entry.
<StructureSection load='1kke' size='340' side='right'caption='[[1kke]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1kke]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KKE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kke OCA], [https://pdbe.org/1kke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kke RCSB], [https://www.ebi.ac.uk/pdbsum/1kke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kke ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SIGM1_REOVD SIGM1_REOVD] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kke_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kke ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1KKE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKE OCA].
*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
==Reference==
__TOC__
Crystal structure of reovirus attachment protein sigma1 reveals evolutionary relationship to adenovirus fiber., Chappell JD, Prota AE, Dermody TS, Stehle T, EMBO J. 2002 Jan 15;21(1-2):1-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11782420 11782420]
</StructureSection>
[[Category: Reovirus sp.]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Mammalian orthoreovirus 3 Dearing]]
[[Category: Chappell, J D.]]
[[Category: Chappell JD]]
[[Category: Dermody, T S.]]
[[Category: Dermody TS]]
[[Category: Prota, A E.]]
[[Category: Prota AE]]
[[Category: Stehle, T.]]
[[Category: Stehle T]]
[[Category: beta-barrel]]
[[Category: beta-spiral]]
[[Category: fiber]]
[[Category: receptor-binding]]
[[Category: reovirus]]
[[Category: sigma1]]
[[Category: trimer]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:07 2008''
Retrieved from "https://proteopedia.org/1kke"