4hem: Difference between revisions

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New page: '''Unreleased structure''' The entry 4hem is ON HOLD Authors: Desmyter, A., Spinelli, S., Farenc, c., Blangy, S., Bebeacua, C., van Sinderen, D., Mahony, J., Cambillau, C Description: ...
 
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'''Unreleased structure'''


The entry 4hem is ON HOLD
==Llama vHH-02 binder of ORF49 (RBP) from lactococcal phage TP901-1==
<StructureSection load='4hem' size='340' side='right'caption='[[4hem]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hem]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_phage_TP901-1 Lactococcus phage TP901-1] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HEM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hem OCA], [https://pdbe.org/4hem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hem RCSB], [https://www.ebi.ac.uk/pdbsum/4hem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hem ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9G096_9CAUD Q9G096_9CAUD]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactococcal phages belong to a large family of Siphoviridae and infect Lactococcus lactis, a gram-positive bacterium used in commercial dairy fermentations. These phages are believed to recognize and bind specifically to pellicle polysaccharides covering the entire bacterium. The phage TP901-1 baseplate, located at the tip of the tail, harbors 18 trimeric receptor binding proteins (RBPs) promoting adhesion to a specific lactococcal strain. Phage TP901-1 adhesion does not require major conformational changes or Ca(2+), which contrasts other lactococcal phages. Here, we produced and characterized llama nanobodies raised against the purified baseplate and the Tal protein of phage TP901-1 as tools to dissect the molecular determinants of phage TP901-1 infection. Using a set of complementary techniques, surface plasmon resonance, EM, and X-ray crystallography in a hybrid approach, we identified binders to the three components of the baseplate, analyzed their affinity for their targets, and determined their epitopes as well as their functional impact on TP901-1 phage infectivity. We determined the X-ray structures of three nanobodies in complex with the RBP. Two of them bind to the saccharide binding site of the RBP and are able to fully neutralize TP901-1 phage infectivity, even after 15 passages. These results provide clear evidence for a practical use of nanobodies in circumventing lactococcal phages viral infection in dairy fermentation.


Authors: Desmyter, A., Spinelli, S., Farenc, c., Blangy, S., Bebeacua, C., van Sinderen, D., Mahony, J., Cambillau, C
Viral infection modulation and neutralization by camelid nanobodies.,Desmyter A, Farenc C, Mahony J, Spinelli S, Bebeacua C, Blangy S, Veesler D, van Sinderen D, Cambillau C Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1371-9. doi:, 10.1073/pnas.1301336110. Epub 2013 Mar 25. PMID:23530214<ref>PMID:23530214</ref>


Description: Llama vHH-02 binder of ORF49 (RBP) from lactococcal phage TP901-1
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hem" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lactococcus phage TP901-1]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Bebeacua C]]
[[Category: Blangy S]]
[[Category: Cambillau C]]
[[Category: Desmyter A]]
[[Category: Farenc C]]
[[Category: Mahony J]]
[[Category: Spinelli S]]
[[Category: Van Sinderen D]]
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