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[[Image:1kne.gif|left|200px]]<br /><applet load="1kne" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1kne, resolution 2.40&Aring;" />
'''Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9'''<br />


==Overview==
==Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9==
The chromodomain of the HP1 family of proteins recognizes histone tails with specifically methylated lysines. Here, we present structural, energetic, and mutational analyses of the complex between the Drosophila HP1 chromodomain and the histone H3 tail with a methyllysine at residue 9, a modification associated with epigenetic silencing. The histone tail inserts as a beta strand, completing the beta-sandwich architecture of the chromodomain. The methylammonium group is caged by three aromatic side chains, whereas adjacent residues form discerning contacts with one face of the chromodomain. Comparison of dimethyl- and trimethyllysine-containing complexes suggests a role for cation-pi and van der Waals interactions, with trimethylation slightly improving the binding affinity.
<StructureSection load='1kne' size='340' side='right'caption='[[1kne]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1kne]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNE FirstGlance]. <br>
1KNE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNE OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kne OCA], [https://pdbe.org/1kne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kne RCSB], [https://www.ebi.ac.uk/pdbsum/1kne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kne ProSAT]</span></td></tr>
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail., Jacobs SA, Khorasanizadeh S, Science. 2002 Mar 15;295(5562):2080-3. Epub 2002 Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11859155 11859155]
</table>
== Function ==
[https://www.uniprot.org/uniprot/HP1_DROME HP1_DROME] Structural component of heterochromatin, involved in gene repression and the modification of position-effect-variegation. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1kne_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kne ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Jacobs, S A.]]
[[Category: Jacobs SA]]
[[Category: Khorasanizadeh, S.]]
[[Category: Khorasanizadeh S]]
[[Category: chromatin]]
[[Category: chromo]]
[[Category: h3]]
[[Category: histone]]
[[Category: hp1]]
[[Category: methyllysine]]
[[Category: trimethyllysine]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:59 2008''

Latest revision as of 08:02, 3 April 2024

Chromo domain of HP1 complexed with histone H3 tail containing trimethyllysine 9

1kne, resolution 2.40Å

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