1i95: Difference between revisions

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[[Image:1i95.png|left|200px]]


{{STRUCTURE_1i95| PDB=1i95 | SCENE= }}
==CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH EDEINE==
<StructureSection load='1i95' size='340' side='right'caption='[[1i95]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1i95]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I95 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDE:EDEINE+B'>EDE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=WO2:OCTADECATUNGSTENYL+DIPHOSPHATE'>WO2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i95 OCA], [https://pdbe.org/1i95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i95 RCSB], [https://www.ebi.ac.uk/pdbsum/1i95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i95 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/1i95_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i95 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The small ribosomal subunit is responsible for the decoding of genetic information and plays a key role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site. Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline. The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA. The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.


===CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH EDEINE===
Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.,Pioletti M, Schlunzen F, Harms J, Zarivach R, Gluhmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F EMBO J. 2001 Apr 17;20(8):1829-39. PMID:11296217<ref>PMID:11296217</ref>


{{ABSTRACT_PUBMED_11296217}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 1i95" style="background-color:#fffaf0;"></div>
[[1i95]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I95 OCA].


==See Also==
==See Also==
*[[Ribosomal protein S10|Ribosomal protein S10]]
*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
*[[Ribosomal protein S11|Ribosomal protein S11]]
*[[Ribosomal protein S12|Ribosomal protein S12]]
*[[Ribosomal protein S13|Ribosomal protein S13]]
*[[Ribosomal protein S14|Ribosomal protein S14]]
*[[Ribosomal protein S15|Ribosomal protein S15]]
*[[Ribosomal protein S16|Ribosomal protein S16]]
*[[Ribosomal protein S17|Ribosomal protein S17]]
*[[Ribosomal protein S18|Ribosomal protein S18]]
*[[Ribosomal protein S19|Ribosomal protein S19]]
*[[Ribosomal protein S2|Ribosomal protein S2]]
*[[Ribosomal protein S20|Ribosomal protein S20]]
*[[Ribosomal protein S3|Ribosomal protein S3]]
*[[Ribosomal protein S4|Ribosomal protein S4]]
*[[Ribosomal protein S5|Ribosomal protein S5]]
*[[Ribosomal protein S6|Ribosomal protein S6]]
*[[Ribosomal protein S7|Ribosomal protein S7]]
*[[Ribosomal protein S8|Ribosomal protein S8]]
*[[Ribosomal protein S9|Ribosomal protein S9]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosome|Ribosome]]
*[[Ribosome|Ribosome]]
 
*[[Ribosome 3D structures|Ribosome 3D structures]]
==Reference==
== References ==
<ref group="xtra">PMID:011296217</ref><references group="xtra"/>
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Avila, H.]]
[[Category: Avila H]]
[[Category: Bartels, H.]]
[[Category: Bartels H]]
[[Category: Franceschi, F.]]
[[Category: Franceschi F]]
[[Category: Gluehmann, M.]]
[[Category: Gluehmann M]]
[[Category: Harms, J.]]
[[Category: Harms J]]
[[Category: Hartsch, T.]]
[[Category: Hartsch T]]
[[Category: Jacobi, C.]]
[[Category: Jacobi C]]
[[Category: Pioletti, M.]]
[[Category: Pioletti M]]
[[Category: Schluenzen, F.]]
[[Category: Schluenzen F]]
[[Category: Yonath, A.]]
[[Category: Yonath A]]
[[Category: Zarivach, R.]]
[[Category: Zarivach R]]
[[Category: 30s ribosome]]
[[Category: Antibiotic]]
[[Category: Edeine]]
[[Category: Ribosome]]
Retrieved from "https://proteopedia.org/1i95"