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[[Image:1lrh.gif|left|200px]]<br /><applet load="1lrh" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1lrh, resolution 1.9&Aring;" />
'''Crystal structure of auxin-binding protein 1 in complex with 1-naphthalene acetic acid'''<br />


==Overview==
==Crystal structure of auxin-binding protein 1 in complex with 1-naphthalene acetic acid==
The structure of auxin-binding protein 1 (ABP1) from maize has been determined at 1.9 A resolution, revealing its auxin-binding site. The structure confirms that ABP1 belongs to the ancient and functionally diverse germin/seed storage 7S protein superfamily. The binding pocket of ABP1 is predominantly hydrophobic with a metal ion deep inside the pocket coordinated by three histidines and a glutamate. Auxin binds within this pocket, with its carboxylate binding the zinc and its aromatic ring binding hydrophobic residues including Trp151. There is a single disulfide between Cys2 and Cys155. No conformational rearrangement of ABP1 was observed when auxin bound to the protein in the crystal, but examination of the structure reveals a possible mechanism of signal transduction.
<StructureSection load='1lrh' size='340' side='right'caption='[[1lrh]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lrh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LRH FirstGlance]. <br>
1LRH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NLA:'>NLA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NLA:NAPHTHALEN-1-YL-ACETIC+ACID'>NLA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lrh OCA], [https://pdbe.org/1lrh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lrh RCSB], [https://www.ebi.ac.uk/pdbsum/1lrh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lrh ProSAT]</span></td></tr>
Crystal structure of auxin-binding protein 1 in complex with auxin., Woo EJ, Marshall J, Bauly J, Chen JG, Venis M, Napier RM, Pickersgill RW, EMBO J. 2002 Jun 17;21(12):2877-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12065401 12065401]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/ABP1_MAIZE ABP1_MAIZE] This is probably a receptor for the plant hormone auxin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lr/1lrh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lrh ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
[[Category: Bauly, J.]]
[[Category: Bauly J]]
[[Category: Chen, J G.]]
[[Category: Chen J-G]]
[[Category: Marshall, J.]]
[[Category: Marshall J]]
[[Category: Napier, R M.]]
[[Category: Napier RM]]
[[Category: Pickersgill, R W.]]
[[Category: Pickersgill RW]]
[[Category: Venis, M.]]
[[Category: Venis M]]
[[Category: Woo, E J.]]
[[Category: Woo EJ]]
[[Category: NLA]]
[[Category: ZN]]
[[Category: beta jellyroll]]
[[Category: double stranded parallel beta helix]]
[[Category: germin like protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:47:40 2008''
Retrieved from "https://proteopedia.org/1lrh"