1m55: Difference between revisions

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[[Image:1m55.gif|left|200px]]<br /><applet load="1m55" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1m55, resolution 1.40&Aring;" />
'''Catalytic domain of the Adeno Associated Virus type 5 Rep protein'''<br />


==Overview==
==Catalytic domain of the Adeno Associated Virus type 5 Rep protein==
Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily.
<StructureSection load='1m55' size='340' side='right'caption='[[1m55]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1m55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_5 Adeno-associated virus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M55 FirstGlance]. <br>
1M55 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M55 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m55 OCA], [https://pdbe.org/1m55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m55 RCSB], [https://www.ebi.ac.uk/pdbsum/1m55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m55 ProSAT]</span></td></tr>
Structural unity among viral origin binding proteins: crystal structure of the nuclease domain of adeno-associated virus Rep., Hickman AB, Ronning DR, Kotin RM, Dyda F, Mol Cell. 2002 Aug;10(2):327-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12191478 12191478]
</table>
[[Category: Adeno-associated virus - 5]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q9YJC1_9VIRU Q9YJC1_9VIRU]  
[[Category: Dyda, F.]]
== Evolutionary Conservation ==
[[Category: Hickman, A B.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kotin, R M.]]
Check<jmol>
[[Category: Ronning, D R.]]
  <jmolCheckbox>
[[Category: CL]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m55_consurf.spt"</scriptWhenChecked>
[[Category: ZN]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: adeno-associated virus]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: endonuclease]]
  </jmolCheckbox>
[[Category: rep]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m55 ConSurf].
[[Category: rolling circle replication]]
<div style="clear:both"></div>
 
__TOC__
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:38 2008''
</StructureSection>
[[Category: Adeno-associated virus 5]]
[[Category: Large Structures]]
[[Category: Dyda F]]
[[Category: Hickman AB]]
[[Category: Kotin RM]]
[[Category: Ronning DR]]
Retrieved from "https://proteopedia.org/1m55"