1v7l: Difference between revisions

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[[Image:1v7l.png|left|200px]]


{{STRUCTURE_1v7l| PDB=1v7l | SCENE= }}
==Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii==
<StructureSection load='1v7l' size='340' side='right'caption='[[1v7l]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1v7l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V7L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v7l OCA], [https://pdbe.org/1v7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v7l RCSB], [https://www.ebi.ac.uk/pdbsum/1v7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v7l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LEUD_PYRHO LEUD_PYRHO] Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v7/1v7l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v7l ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recent studies have implied that the isopropylmalate isomerase small subunit of the hyperthermophilic archaea Pyrococcus horikoshii (PhIPMI-s) functions as isopropylmalate isomerase in the leucine biosynthesis pathway, and as homoaconitase (HACN) in the lysine biosynthesis pathway via alpha-aminoadipic acid. PhIPMI is thus considered a key to understanding the fundamental metabolism of the earliest organisms. We describe for the first time the crystal structure of PhIPMI-s, which displays dual substrate specificity. The crystal structure unexpectedly shows that four molecules create an interlocked assembly with intermolecular disulfide linkages having a skewed 222 point-group symmetry. Although the overall fold of the PhIPMI-s monomer is related closely to domain 4 of the aconitase (ACN), one alpha-helix in the ACN structure is replaced by a short loop with relatively high temperature factor values. Because this region is essential for discriminating the structurally similar substrate based on interactions with its diversified gamma-moiety, the loop structure in the PhIPMI-s must be dependent on the presence of a substrate. The flexibility of the loop region might be a structural basis for recognizing both hydrophobic and hydrophilic gamma-moieties of two distinct substrates, isopropylmalate and homocitrate.


===Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii===
Crystal structure of the Pyrococcus horikoshii isopropylmalate isomerase small subunit provides insight into the dual substrate specificity of the enzyme.,Yasutake Y, Yao M, Sakai N, Kirita T, Tanaka I J Mol Biol. 2004 Nov 19;344(2):325-33. PMID:15522288<ref>PMID:15522288</ref>


{{ABSTRACT_PUBMED_15522288}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 1v7l" style="background-color:#fffaf0;"></div>
[[1v7l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7L OCA].


==See Also==
==See Also==
*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
 
*[[Isopropylmalate isomerase|Isopropylmalate isomerase]]
==Reference==
== References ==
<ref group="xtra">PMID:015522288</ref><references group="xtra"/>
<references/>
[[Category: 3-isopropylmalate dehydratase]]
__TOC__
[[Category: Pyrococcus horikoshii]]
</StructureSection>
[[Category: Kirita, T.]]
[[Category: Large Structures]]
[[Category: Sakai, N.]]
[[Category: Pyrococcus horikoshii OT3]]
[[Category: Tanaka, I.]]
[[Category: Kirita T]]
[[Category: Yao, M.]]
[[Category: Sakai N]]
[[Category: Beta barrel]]
[[Category: Tanaka I]]
[[Category: Lyase]]
[[Category: Yao M]]
Retrieved from "https://proteopedia.org/1v7l"