1n5z: Difference between revisions

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[[Image:1n5z.gif|left|200px]]<br /><applet load="1n5z" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1n5z, resolution 2.70&Aring;" />
'''Complex structure of Pex13p SH3 domain with a peptide of Pex14p'''<br />


==Overview==
==Complex structure of Pex13p SH3 domain with a peptide of Pex14p==
While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.
<StructureSection load='1n5z' size='340' side='right'caption='[[1n5z]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1n5z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N5Z FirstGlance]. <br>
1N5Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5z OCA], [https://pdbe.org/1n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n5z RCSB], [https://www.ebi.ac.uk/pdbsum/1n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5z ProSAT]</span></td></tr>
==Reference==
</table>
Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain., Douangamath A, Filipp FV, Klein AT, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M, Mol Cell. 2002 Nov;10(5):1007-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12453410 12453410]
== Function ==
[[Category: Protein complex]]
[https://www.uniprot.org/uniprot/PEX13_YEAST PEX13_YEAST] Component of the peroxisomal translocation machinery with PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5z ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Barnett, P.]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Distel, B.]]
[[Category: Barnett P]]
[[Category: Douangamath, A.]]
[[Category: Distel B]]
[[Category: Filipp, F V.]]
[[Category: Douangamath A]]
[[Category: Klein, A T.J.]]
[[Category: Filipp FV]]
[[Category: Mayans, O M.]]
[[Category: Klein ATJ]]
[[Category: Sattler, M.]]
[[Category: Mayans OM]]
[[Category: Vega, M C.]]
[[Category: Sattler M]]
[[Category: Voorn-Brouwer, T.]]
[[Category: Vega MC]]
[[Category: Wilmanns, M.]]
[[Category: Voorn-Brouwer T]]
[[Category: Zou, P.]]
[[Category: Wilmanns M]]
[[Category: pxxp motif]]
[[Category: Zou P]]
[[Category: sh3 domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:34 2008''

Latest revision as of 13:27, 13 March 2024

Complex structure of Pex13p SH3 domain with a peptide of Pex14p

1n5z, resolution 2.70Å

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