1a5a: Difference between revisions

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[[Image:1a5a.gif|left|200px]]<br />
<applet load="1a5a" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1a5a, resolution 1.9&Aring;" />
'''CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHAD60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49'''<br />


==Overview==
==CRYO-CRYSTALLOGRAPHY OF A TRUE SUBSTRATE, INDOLE-3-GLYCEROL PHOSPHATE, BOUND TO A MUTANT (ALPHAD60N) TRYPTOPHAN SYNTHASE ALPHA2BETA2 COMPLEX REVEALS THE CORRECT ORIENTATION OF ACTIVE SITE ALPHA GLU 49==
The reversible cleavage of indole-3-glycerol by the alpha-subunit of, tryptophan synthase has been proposed to be catalyzed by alphaGlu49 and, alphaAsp60. Although previous x-ray crystallographic structures of the, tryptophan synthase alpha2beta2 complex showed an interaction between the, carboxylate of alphaAsp60 and the bound inhibitor indole-3-propanol, phosphate, the carboxylate of alphaGlu49 was too distant to play its, proposed role. To clarify the structural and functional roles of, alphaGlu49, we have determined crystal structures of a mutant (alphaD60N), alpha2beta2 complex in the presence and absence of the true substrate, indole-3-glycerol phosphate. The enzyme in the crystal cleaves, indole-3-glycerol phosphate very slowly at room temperature but not under, cryo-conditions of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9535826 (full description)]]
<StructureSection load='1a5a' size='340' side='right'caption='[[1a5a]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1a5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5a OCA], [https://pdbe.org/1a5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5a RCSB], [https://www.ebi.ac.uk/pdbsum/1a5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRPA_SALTY TRPA_SALTY] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5a ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1A5A is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with K and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Site: PLP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5A OCA]].
*[[Tryptophan synthase 3D structures|Tryptophan synthase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Cryo-crystallography of a true substrate, indole-3-glycerol phosphate, bound to a mutant (alphaD60N) tryptophan synthase alpha2beta2 complex reveals the correct orientation of active site alphaGlu49., Rhee S, Miles EW, Davies DR, J Biol Chem. 1998 Apr 10;273(15):8553-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9535826 9535826]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Salmonella typhimurium]]
[[Category: Davies DR]]
[[Category: Tryptophan synthase]]
[[Category: Miles EW]]
[[Category: Davies, D.R.]]
[[Category: Rhee S]]
[[Category: Miles, E.W.]]
[[Category: Rhee, S.]]
[[Category: K]]
[[Category: PLP]]
[[Category: carbon-oxygen lyase]]
[[Category: mutation at position 60 (asp --> asn) in the a-subunit]]
 
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Retrieved from "https://proteopedia.org/1a5a"