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[[Image:1bxi.png|left|200px]]


{{STRUCTURE_1bxi|  PDB=1bxi  |  SCENE=  }}
==CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9==
 
<StructureSection load='1bxi' size='340' side='right'caption='[[1bxi]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
===CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1bxi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BXI FirstGlance]. <br>
{{ABSTRACT_PUBMED_010074943}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bxi OCA], [https://pdbe.org/1bxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bxi RCSB], [https://www.ebi.ac.uk/pdbsum/1bxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bxi ProSAT]</span></td></tr>
[[1bxi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXI OCA].  
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/1bxi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bxi ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Colicin|Colicin]]
*[[Colicin 3D structures|Colicin 3D structures]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
*[[Colicin Immunity Protein 7|Colicin Immunity Protein 7]]
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
*[[Colicin Immunity Protein 9|Colicin Immunity Protein 9]]
__TOC__
*[[Directed evolution|Directed evolution]]
</StructureSection>
*[[Mutant immunity protein 9 (variant R12-13)|Mutant immunity protein 9 (variant R12-13)]]
*[[Mutant immunity protein 9 (variant R12-2)|Mutant immunity protein 9 (variant R12-2)]]
 
==Reference==
<ref group="xtra">PMID:010074943</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Hemmings, A M.]]
[[Category: Large Structures]]
[[Category: James, R.]]
[[Category: Hemmings AM]]
[[Category: Kleanthous, C.]]
[[Category: James R]]
[[Category: Kuhlmann, U C.]]
[[Category: Kleanthous C]]
[[Category: Moore, G R.]]
[[Category: Kuhlmann UC]]
[[Category: Colicin]]
[[Category: Moore GR]]
[[Category: Endonuclease]]
[[Category: Immune system]]
[[Category: Protein-protein interaction]]
Retrieved from "https://proteopedia.org/1bxi"