1cfl: Difference between revisions
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==DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT== | |||
<StructureSection load='1cfl' size='340' side='right'caption='[[1cfl]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1cfl]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=64T:5-HYDROXY-THYMIDINE-5-MONOPHOSPHATE'>64T</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfl OCA], [https://pdbe.org/1cfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfl RCSB], [https://www.ebi.ac.uk/pdbsum/1cfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfl ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --> C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --> C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --> C transition. | |||
Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct.,Lee JH, Hwang GS, Choi BS Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:10359763<ref>PMID:10359763</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | <div class="pdbe-citations 1cfl" style="background-color:#fffaf0;"></div> | ||
[[ | == References == | ||
[[Category: Choi | <references/> | ||
[[Category: Hwang | __TOC__ | ||
[[Category: Lee | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Choi B-S]] | |||
[[Category: Hwang G-S]] | |||
[[Category: Lee J-H]] | |||
Latest revision as of 11:39, 22 November 2023
DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
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