1p65: Difference between revisions

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[[Image:1p65.jpg|left|200px]]<br /><applet load="1p65" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1p65, resolution 2.60&Aring;" />
'''Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)'''<br />


==Overview==
==Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)==
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus of the Arteriviridae family, genomically related to the coronaviruses. PRRSV is the causative agent of both severe and persistent respiratory disease and reproductive failure in pigs worldwide. The PRRSV virion contains a core made of the 123 amino acid nucleocapsid (N) protein, a product of the ORF7 gene. We have determined the crystal structure of the capsid-forming domain of N. The structure was solved to 2.6 A resolution by SAD methods using the anomalous signal from sulfur. The N protein exists in the crystal as a tight dimer forming a four-stranded beta sheet floor superposed by two long alpha helices and flanked by two N- and two C-terminal alpha helices. The structure of N represents a new class of viral capsid-forming domains, distinctly different from those of other known enveloped viruses, but reminiscent of the coat protein of bacteriophage MS2.
<StructureSection load='1p65' size='340' side='right'caption='[[1p65]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1p65]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_reproductive_and_respiratory_syndrome_virus Porcine reproductive and respiratory syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P65 FirstGlance]. <br>
1P65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Porcine_respiratory_and_reproductive_syndrome_virus Porcine respiratory and reproductive syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P65 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p65 OCA], [https://pdbe.org/1p65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p65 RCSB], [https://www.ebi.ac.uk/pdbsum/1p65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p65 ProSAT]</span></td></tr>
==Reference==
</table>
Structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus., Doan DN, Dokland T, Structure. 2003 Nov;11(11):1445-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14604534 14604534]
== Function ==
[[Category: Porcine respiratory and reproductive syndrome virus]]
[https://www.uniprot.org/uniprot/Q9YJI1_PRRSV Q9YJI1_PRRSV]  
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Doan, D N.P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Dokland, T.]]
Check<jmol>
[[Category: nucleocapsid]]
  <jmolCheckbox>
[[Category: virus]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p65_consurf.spt"</scriptWhenChecked>
 
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:31 2008''
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p65 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Porcine reproductive and respiratory syndrome virus]]
[[Category: Doan DNP]]
[[Category: Dokland T]]
Retrieved from "https://proteopedia.org/1p65"