Barnase: Difference between revisions

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[[Image:1bni.png|left|200px|thumb|Crystal Structure of Barnase [[1bni]]]]
<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
{{STRUCTURE_1bni|  PDB=1bni  | SIZE=400| SCENE=Barnase/Cv/1 |right|CAPTION=Barnase [[1bni]] }}


== Function ==


'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.


== Relevance ==


BA structure was used extensively for studying protein folding.


== Structural highlights ==


 
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
 
 
 
 
 
 
 
 
 
 
 
 
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''.  The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
 
{{TOC limit|limit=2}}


== 3D Structures of Barnase ==
== 3D Structures of Barnase ==
[[Barnase 3D structures]]


[[3kch]], [[2f4y]] – BA cross-linked<br />
</StructureSection>
[[2f56]] - BA cross-linked + urea<br />
[[2f5w]] - BA cross-linked + thiourea<br />
[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
[[1fw7]], [[1bnr]] – BA - NMR<br />
[[2kf3]] – BA (mutant) – NMR<br />
[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
[[1yvs]] – BA domain swapped<br />
[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
 
===BA + BST===
 
[[3da7]] - BA (mutant) + BST<br />
[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
 
[[2c4b]] – BA/MCOEETI (mutant)


== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
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