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[[Image:4aik.png|left|200px]]


{{STRUCTURE_4aik| PDB=4aik | SCENE= }}
==Crystal structure of RovA from Yersinia in complex with an invasin promoter fragment==
<StructureSection load='4aik' size='340' side='right'caption='[[4aik]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4aik]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AIK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aik OCA], [https://pdbe.org/4aik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aik RCSB], [https://www.ebi.ac.uk/pdbsum/4aik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aik ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SLYA_YERPY SLYA_YERPY] Transcription regulator that can specifically activate or repress expression of target genes.[HAMAP-Rule:MF_01819]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pathogens often rely on thermosensing to adjust virulence gene expression. In yersiniae, important virulence-associated traits are under the control of the master regulator RovA which uses an in-built thermosensor to control its activity. Thermal upshifts encountered upon host entry induce conformational changes of the RovA dimer that attenuate DNA-binding and render the protein more susceptible to proteolysis. Here, we report the crystal structure of RovA in the free and DNA-bound form and provide evidence that thermo-induced loss of RovA activity is mainly promoted by a thermosensing loop in the dimerization domain and residues in the adjacent C-terminal helix. These determinants allow partial unfolding of the regulator upon an upshift to 37 degrees C. This structural distortion is transmitted to the flexible DNA-binding domain of RovA. RovA mainly contacts the DNA backbone in a low affinity-binding mode which allows the immediate release of RovA from its operator sites. We also show that SlyA, a close homologue of RovA from Salmonella with a very similar structure, is not a thermosensor and remains active and stable at 37 degrees C. Strikingly, changes in only three amino acids, reflecting evolutionary replacements in SlyA, result in a complete loss of the thermosensing properties of RovA and prevent degradation. In conclusion, only minor alterations can transform a thermotolerant regulator into a thermosensor that allows adjustment of virulence and fitness determinants to their thermal environment.


===Crystal structure of RovA from Yersinia in complex with an invasin promoter fragment===
Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.,Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P J Biol Chem. 2012 Aug 30. PMID:22936808<ref>PMID:22936808</ref>


{{ABSTRACT_PUBMED_22936808}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4aik" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[4aik]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIK OCA].
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Yersinia pseudotuberculosis]]
[[Category: Yersinia pseudotuberculosis]]
[[Category: Dersch, P.]]
[[Category: Dersch P]]
[[Category: Heinz, D W.]]
[[Category: Heinz DW]]
[[Category: Herbst, K.]]
[[Category: Herbst K]]
[[Category: Heroven, A K.]]
[[Category: Heroven AK]]
[[Category: Mendonca, C.]]
[[Category: Mendonca C]]
[[Category: Quade, N.]]
[[Category: Quade N]]
[[Category: Global regulator]]
[[Category: Thermosensing]]
[[Category: Transcription]]
[[Category: Transcription factor]]
[[Category: Virulence regulation]]
Retrieved from "https://proteopedia.org/4aik"