Sandbox Reserved 640: Difference between revisions
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#The Rhizobium bacteroid <ref name=b>Appleby, C. A. "Leghemoglobin and Rhizobium Respiration." Annual Review of Plant Physiology 35.1 (1984): 443-78. Print.</ref> is thought to synthesize the heme group in the root nodules of the legume, where it then gives the plant the heme to complete the synthesizing of the Lb <ref name=a /> | #The Rhizobium bacteroid <ref name=b>Appleby, C. A. "Leghemoglobin and Rhizobium Respiration." Annual Review of Plant Physiology 35.1 (1984): 443-78. Print.</ref> is thought to synthesize the heme group in the root nodules of the legume, where it then gives the plant the heme to complete the synthesizing of the Lb <ref name=a /> | ||
#The plant itself has also been thought to possibly produce the heme itself, in the mytochondria of the plant cells. It then combines with the peptide sequence to complete the whole protein <ref name=a /> | #The plant itself has also been thought to possibly produce the heme itself, in the mytochondria of the plant cells. It then combines with the peptide sequence to complete the whole protein <ref name=a /> | ||
==Function== | ==Function== | ||
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*Isoelectrofocusing | *Isoelectrofocusing | ||
*Nuclear Magnetic Resonance (NMR) | *Nuclear Magnetic Resonance (NMR) | ||
*X-ray crystallography | *X-ray crystallography | ||
==Mechanism== | ==Mechanism== | ||
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<div style="text-align: center;"> NAD(P)H + H+ + 2 ferrileghemoglobin NAD(P)+ + 2 ferroleghemoglobin </div> | <div style="text-align: center;"> NAD(P)H + H+ + 2 ferrileghemoglobin NAD(P)+ + 2 ferroleghemoglobin </div> | ||
Ferric Leghemoglobin can also be reduced to the ferrous form by free flavins in the presence of NADH or NADPH. Lastly, Leghemoglobin can be reduced to its ferrous form directly by physiological reductants. These reductants are various electron donors commonly found in plant cells, which reduce the Leghemoglobin nonenzymatically.<ref name=b/>. | Ferric Leghemoglobin can also be reduced to the ferrous form by free flavins in the presence of NADH or NADPH. Lastly, Leghemoglobin can be reduced to its ferrous form directly by physiological reductants. These reductants are various electron donors commonly found in plant cells, which reduce the Leghemoglobin nonenzymatically.<ref name=b/>. | ||
==Implications and Applications== | ==Implications and Applications== | ||
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Other nitrogen fixing plants don’t need oxygen, or need very trace amounts, for nitrogen fixation. Some need heterocysts, which are specific nitrogen-fixing cells, and have no need for Leghemoglobin because of its own anti-oxygen evolved state. Examples of what could replace Leghemoglobins are proteins that are specific to scavenging oxygen out of the cells, as well as having multiple cell walls, one of which is a glycolipid, that helps to keep out oxygen <ref name=ten />. | Other nitrogen fixing plants don’t need oxygen, or need very trace amounts, for nitrogen fixation. Some need heterocysts, which are specific nitrogen-fixing cells, and have no need for Leghemoglobin because of its own anti-oxygen evolved state. Examples of what could replace Leghemoglobins are proteins that are specific to scavenging oxygen out of the cells, as well as having multiple cell walls, one of which is a glycolipid, that helps to keep out oxygen <ref name=ten />. | ||
Nitrogen Fixation is essential for Crop Rotation, as some plants need nitrogen and others assist in nitrogen fixation that can provide it. The legumes play an important role in this as they can be rotated with vegetable crops that need a renewable nitrogen source. Maize is one of these crops that is perfect to rotate with soybeans to maintain healthy soil. Otherwise, the corn would diminish the nitrogen and the land would be unable to grow more. The soybeans are grown on the same soil afterwards to replenish the nitrogen. The corn is then rotated back in to have a fruitful harvest. | |||
==Notes== | ==Notes== | ||
{{reflist}} | {{reflist}} | ||