Nitrate reductase: Difference between revisions

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{{STRUCTURE_2jim|  PDB=2jim  | SIZE=400| SCENE= |right|CAPTION=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo ion and molybdepterin guanine nucleotide, [[2jim]] }}
<StructureSection load='' size='350' side='right' caption='Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry [[2jiq]])' scene='49/490062/Cv/3'>


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== Function ==
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'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>.  NR active site contains Mo atom.  Four types of NR are known:<br />
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'''eukaryotic assimilatory NR'''<br />
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'''bacterial cytoplasmic assimilatory''' (NAC)<br />
-->
'''bacterial membrane-bound respiratory''' (NAR)<br />
'''Nitrate reductase''' (NR) catalyzes the reduction of NO3 to NO2 using NADPH .  NR active site contains Mo atom.  Four types of NR are known: one eukaryotic assimilatory NR and 3 bacterial NRs – cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) and periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
'''bacterial periplasmic dissimilatory''' (NAP).<br />
The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
 
== Structural highlights ==
<scene name='49/490062/Cv/7'>Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides</scene>. NAP active site includes a <scene name='49/490062/Cv/8'>six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140</scene>. The <scene name='49/490062/Cv/9'>NO3 molecule is guided to the active site by a funnel leading to the Mo atom</scene><ref>PMID:18327621</ref>.
 
</StructureSection>


==3D structures of nitrate reductase==
==3D structures of nitrate reductase==


'''Bacterial periplasmic dissimilatory NR'''
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Bacterial periplasmic dissimilatory NR
 
**[[2nap]], [[2jim]], [[2jio]], [[2jip]], [[2v3v]], [[2v45]] – DdNAP + MGD + Mo - ''Desulfovibrio desulfuricans''<br />
**[[2jiq]] - DdNAP + NO3 + MGD + Mo<br />
**[[2jir]] - DdNAP + CN + MGD + Mo<br />
**[[1jni]] – NAP small subunit + heme – ''Haemophilus influenzae''<br />
**[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
**[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
**[[3ml1]], [[3o5a]] - NAP catalytic subunit  + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''<br />
**[[5yly]] – NR cytochrome b5 reductase domain + FAD – ''Ulva prolifera''<br />
 
*Bacterial respiratory NR


[[2nap]], [[2jim]], [[2jio]], [[2jip]], [[2v3v]], [[2v45]] – DdNAP + MGD + Mo - ''Desulfovibrio desulfuricans''<br />  
**[[1q16]], [[1y5l]] – EcNAR α+β+γ chains + heme + MGD + Mo<br />  
[[2jiq]] - DdNAP + NO3 + MGD + Mo<br />
**[[1r27]] - EcNAR α+β chains + MGD + Mo<br />
[[2jir]] - DdNAP + CN + MGD + Mo<br />
**[[1siw]] – EcNAR α+β+γ chains + heme<br />
[[1jni]] – NAP small subunit + heme – ''Haemophilus influenzae''<br />
**[[1y4z]], [[3egw]] - EcNAR α+β (mutant) + γ chains + inhibitor + heme + MGD + Mo<br />
[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
**[[1y5i]], [[1y5n]] - EcNAR α+β + γ (mutant) chains + inhibitor + heme + MGD + Mo<br />
[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
**[[3ir5]], [[3ir7]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + MGD + Mo<br />
[[3ml1]], [[3o5a]] - NAP catalytic subunit  + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''
**[[3ir6]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + GDP


'''Bacterial respiratory NR'''
*Eukaryotic assimilatory NR


[[1q16]] – EcNAR α+β+γ chains + heme + MGD + Mo<br />
**[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
[[1r27]] - EcNAR α+β chains + MGD + Mo<br />
[[1siw]] – EcNAR α+β+γ chains + heme<br />
[[1y4z]], [[3egw]] - EcNAR α+β (mutant) + γ chains + inhibitor + heme + MGD + Mo<br />
[[1y5i]], [[1y5n]] - EcNAR α+β + γ (mutant) chains + inhibitor + heme + MGD + Mo<br />
[[3ir5]], [[3ir7]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + MGD + Mo<br />
[[3ir6]] - EcNAR α (mutant) +β + γ chains + inhibitor + heme + GDP


'''Eukaryotic assimilatory NR'''
*Eukaryotic NR


[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
**[[1cne]] – cNR + FAD – corn<br />
**[[1cnf]], [[2cnd]] – cNR (mutant) + FAD + ADP<br />


}}
== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 13:48, 23 July 2019

Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry 2jiq)

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3D structures of nitrate reductase

Updated on 23-July-2019

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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