4hvn: Difference between revisions
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New page: '''Unreleased structure''' The entry 4hvn is ON HOLD Authors: Filippova, E.V., Minasov, G., Shuvalova, L., Kiryukhina, O., Jedrzejczak, R., Joachimiak, A., Anderson, W.F., Midwest Cente... |
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==Crystal structure of hypothetical protein with ketosteroid isomerase-like protein fold from Catenulispora acidiphila DSM 44928 in complex with Trimethylamine.== | |||
<StructureSection load='4hvn' size='340' side='right'caption='[[4hvn]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hvn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catenulispora_acidiphila_DSM_44928 Catenulispora acidiphila DSM 44928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HVN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KEN:N,N-DIMETHYLMETHANAMINE'>KEN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hvn OCA], [https://pdbe.org/4hvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hvn RCSB], [https://www.ebi.ac.uk/pdbsum/4hvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hvn ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/C7PVH9_CATAD C7PVH9_CATAD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Catenulispora acidiphila is a newly identified lineage of actinomycetes that produces antimicrobial activities and represents a promising source of novel antibiotics and secondary metabolites. Among the discovered protein coding genes, 68 % were assigned a putative function, while the remaining 32 % are genes encoding "hypothetical" proteins. Caci_0382 is one of the "hypothetical" proteins that has very few homologs. Sequence analysis shows that the protein belongs to the NTF2-like protein family. The structure of Caci_0382 demonstrates that it shares the same fold and has a similar active site as limonene-1,2-epoxide hydrolase, which suggests that it may have a related function. Using a fluorescence thermal shift assay, we identified stabilizing compounds that suggest potential natural ligands of Caci_0382. Using this information, we determined the crystal structure in complex with trimethylamine to provide a better understanding of the function of this uncharacterized protein. | |||
Structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in Catenulispora acidiphila.,Filippova EV, Luan CH, Dunne SF, Kiryukhina O, Minasov G, Shuvalova L, Anderson WF J Struct Funct Genomics. 2014 Mar;15(1):33-40. doi: 10.1007/s10969-014-9176-z., Epub 2014 Feb 22. PMID:24562475<ref>PMID:24562475</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4hvn" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Catenulispora acidiphila DSM 44928]] | |||
[[Category: Large Structures]] | |||
[[Category: Anderson WF]] | |||
[[Category: Filippova EV]] | |||
[[Category: Jedrzejczak R]] | |||
[[Category: Joachimiak A]] | |||
[[Category: Kiryukhina O]] | |||
[[Category: Minasov G]] | |||
[[Category: Shuvalova L]] | |||
Latest revision as of 15:11, 20 September 2023
Crystal structure of hypothetical protein with ketosteroid isomerase-like protein fold from Catenulispora acidiphila DSM 44928 in complex with Trimethylamine.
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