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[[Image:1q3a.jpg|left|200px]]<br /><applet load="1q3a" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1q3a, resolution 2.10&Aring;" />
'''Crystal structure of the catalytic domain of human matrix metalloproteinase 10'''<br />


==Overview==
==Crystal structure of the catalytic domain of human matrix metalloproteinase 10==
<StructureSection load='1q3a' size='340' side='right'caption='[[1q3a]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1q3a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q3A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NGH:N-ISOBUTYL-N-[4-METHOXYPHENYLSULFONYL]GLYCYL+HYDROXAMIC+ACID'>NGH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q3a OCA], [https://pdbe.org/1q3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q3a RCSB], [https://www.ebi.ac.uk/pdbsum/1q3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q3a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MMP10_HUMAN MMP10_HUMAN] Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q3/1q3a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q3a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The catalytic domain of matrix metalloproteinase-10 (MMP-10) has been expressed in Escherichia coli and its crystal structure solved at 2.1 A resolution. The availability of this structure allowed us to critically examine the small differences existing between the catalytic domains of MMP-3 and MMP-10, which show the highest sequence identity among all MMPs. Furthermore, the binding mode of N-isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH), which is one of the most known commercial inhibitors of MMPs, is described for the first time.
The catalytic domain of matrix metalloproteinase-10 (MMP-10) has been expressed in Escherichia coli and its crystal structure solved at 2.1 A resolution. The availability of this structure allowed us to critically examine the small differences existing between the catalytic domains of MMP-3 and MMP-10, which show the highest sequence identity among all MMPs. Furthermore, the binding mode of N-isobutyl-N-[4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH), which is one of the most known commercial inhibitors of MMPs, is described for the first time.


==About this Structure==
Crystal structure of the catalytic domain of human matrix metalloproteinase 10.,Bertini I, Calderone V, Fragai M, Luchinat C, Mangani S, Terni B J Mol Biol. 2004 Feb 20;336(3):707-16. PMID:15095982<ref>PMID:15095982</ref>
1Q3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=NGH:'>NGH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Stromelysin_2 Stromelysin 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.22 3.4.24.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3A OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the catalytic domain of human matrix metalloproteinase 10., Bertini I, Calderone V, Fragai M, Luchinat C, Mangani S, Terni B, J Mol Biol. 2004 Feb 20;336(3):707-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15095982 15095982]
</div>
<div class="pdbe-citations 1q3a" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Matrix metalloproteinase 3D structures|Matrix metalloproteinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Stromelysin 2]]
[[Category: Bertini I]]
[[Category: Bertini, I.]]
[[Category: Calderone V]]
[[Category: Calderone, V.]]
[[Category: Fragai M]]
[[Category: Fragai, M.]]
[[Category: Luchinat C]]
[[Category: Luchinat, C.]]
[[Category: Mangani S]]
[[Category: Mangani, S.]]
[[Category: Terni B]]
[[Category: Terni, B.]]
[[Category: CA]]
[[Category: NGH]]
[[Category: ZN]]
[[Category: hydroxamic acid]]
[[Category: inhibitors]]
[[Category: metalloproteinase]]
[[Category: mmp-10]]
[[Category: nngh]]
[[Category: stromelysin-2]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:35:26 2008''
Retrieved from "https://proteopedia.org/1q3a"