1q7o: Difference between revisions

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[[Image:1q7o.jpg|left|200px]]<br /><applet load="1q7o" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1q7o" />
'''Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy'''<br />


==Overview==
==Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy==
<StructureSection load='1q7o' size='340' side='right'caption='[[1q7o]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1q7o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q7O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=MTY:META-TYROSINE'>MTY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q7o OCA], [https://pdbe.org/1q7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q7o RCSB], [https://www.ebi.ac.uk/pdbsum/1q7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q7o ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising.
The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising.


==About this Structure==
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy.,Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:12149447<ref>PMID:12149447</ref>
1Q7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7O OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy., Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12149447 12149447]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1q7o" style="background-color:#fffaf0;"></div>
[[Category: Griffin, R G.]]
== References ==
[[Category: Hohwy, M.]]
<references/>
[[Category: Jaroniec, C P.]]
__TOC__
[[Category: Lozano-Perez, T.]]
</StructureSection>
[[Category: McMahon, M T.]]
[[Category: Large Structures]]
[[Category: Reif, B.]]
[[Category: Griffin RG]]
[[Category: Rienstra, C M.]]
[[Category: Hohwy M]]
[[Category: Tidor, B.]]
[[Category: Jaroniec CP]]
[[Category: Tucker-Kellogg, L.]]
[[Category: Lozano-Perez T]]
[[Category: chemotactic peptide]]
[[Category: McMahon MT]]
[[Category: mlf]]
[[Category: Reif B]]
 
[[Category: Rienstra CM]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:42 2008''
[[Category: Tidor B]]
[[Category: Tucker-Kellogg L]]
Retrieved from "https://proteopedia.org/1q7o"