Transketolase: Difference between revisions

Latest revision as of 13:54, 4 January 2023

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E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code 2r8o).

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References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Verschueren KH, Kingma J, Rozeboom HJ, Kalk KH, Janssen DB, Dijkstra BW. Crystallographic and fluorescence studies of the interaction of haloalkane dehalogenase with halide ions. Studies with halide compounds reveal a halide binding site in the active site. Biochemistry. 1993 Sep 7;32(35):9031-7. PMID:8369276

[2] James P, Isupov MN, De Rose SA, Sayer C, Cole IS, Littlechild JA. A 'Split-Gene' Transketolase From the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization. Front Microbiol. 2020 Oct 30;11:592353. doi: 10.3389/fmicb.2020.592353., eCollection 2020. PMID:33193259 doi:https://dx.doi.org/10.3389/fmicb.2020.592353

[3] Asztalos P, Parthier C, Golbik R, Kleinschmidt M, Hubner G, Weiss MS, Friedemann R, Wille G, Tittmann K. Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate. Biochemistry. 2007 Oct 30;46(43):12037-52. Epub 2007 Oct 3. PMID:17914867 doi:10.1021/bi700844m

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-{{STRUCTURE_3m49| PDB=3m49  | SIZE=400| SCENE= |right|CAPTION=Human transketolase complex with co-factor thiamine diphosphate, ethanediol, ethylene glycol and Ca+2, Na+ ions [[3m49]] }}
+<StructureSection load='' size='350' side='right' caption='E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green)  (PDB code [[2r8o]]).' scene='46/466534/Cv/1'>
+== Function ==
+'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the [[Calvin cycle]]<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.
-'''Transketolase''' (TKT) catalyzes two opposite reactions.  The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle.
+== Structural highlights ==
-{{TOC limit|limit=2}}
+The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.
 == 3D Structures of transketolase ==
-''Update July 2012''
+[[Transketolase 3D structures]]
+</StructureSection>
-[[1trk]] – yTKT – yeast
-[[1ay0]] – yTKT (mutant)
-[[2e6k]] – TKT – ''Thermus thermophilus''
-[[3hyl]] – BaTKT – ''Bacillus anthracis''
-[[3kom]] – TKT (mutant) – ''Francisella tularensis''
-[[3l84]] – CjTKT – ''Campylobacter jejuni''<br />
-[[3rim]] – TKT – ''Mycobacterium tuberculosis''<br />
-[[3uk1]] – BtTKT – ''Burkholderia thailandensis''
-===Transketolase binary complexes===
-[[3m49]] – BaTKT + TPP
-[[3mos]], [[3ooy]] – TKT + TPP - human
-[[1tka]], [[1tkb]], [[1tkc]] – yTKT + TPP analog
-[[1gpu]] - yTKT + TPP derivative
-[[1itz]] - TKT + TPP – maize
-[[1qgd]] - EcTKT + TPP – ''Escherichia coli''
-[[1r9j]] - TKT + TPP – ''Leishmania mexicana''
-===Transketolase ternary complexes===
-[[3m7i]], [[3m6l]] – CjTKT + TPP + ribose 5-P
-[[3m34]] - CjTKT + TPP + Ca
-[[1ngs]] - yTKT + TPP + Ca + erythrose-4-P
-[[2r5n]] – EcTKT + TPP + ribose 5-P<br />
-[[3upt]] - BtTKT + TPP + ribose 5-P<br />
-[[2r8o]] - EcTKT + TPP + xylulose 5-P
-[[2r8p]] - EcTKT + TPP + fructose 6-P
+== References ==
+<references/>
 [[Category:Topic Page]]

Transketolase: Difference between revisions

Latest revision as of 13:54, 4 January 2023

Function

Structural highlights

3D Structures of transketolase

References

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