4hu8: Difference between revisions

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'''Unreleased structure'''


The entry 4hu8 is ON HOLD  until Paper Publication
==Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut==
<StructureSection load='4hu8' size='340' side='right'caption='[[4hu8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hu8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Globitermes_brachycerastes Globitermes brachycerastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HU8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hu8 OCA], [https://pdbe.org/4hu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hu8 RCSB], [https://www.ebi.ac.uk/pdbsum/4hu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hu8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K7PDC1_9BACT K7PDC1_9BACT]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial Ig-like (Big) domains are commonly distributed in glycoside hydrolases (GH), but their structure and function remains undefined. Xylanase is a GH, and catalyzes the hydrolysis of the internal beta-xylosidic linkages of xylan. In this study, we report the molecular cloning, biochemical and biophysical characterization, and crystal structure of a termite gut bacterial xylanase, Xyl-ORF19, which was derived from gut bacteria of a wood-feeding termite (Globitermes brachycerastes). The protein architecture of Xyl-ORF19 reveals that it has two domains, a C-terminal GH10 catalytic domain and an N-terminal Big_2 non-catalytic domain. The catalytic domain folds in an (alpha/beta)8 barrel as most GH10 xylanases do, but it has two extra beta-strands. The non-catalytic domain is structurally similar to an immunoglobulin-like domain of intimins. The recombinant enzyme without the non-catalytic domain has fairly low catalytic activity, and is different from the full-length enzyme in kinetic parameters, pH and temperature profiles, which suggests the non-catalytic domain could affect the enzyme biochemical and biophysical properties as well as the role for enzyme localization. This study provides a molecular basis for future efforts in xylanase bioengineering. Biotechnol. Bioeng. 2013;9999: 1-11. (c) 2013 Wiley Periodicals, Inc.


Authors: Han, Q., Liu, N., Robinson, H., Cao, L., Qian, C., Wang, Q., Xie, L., Ding, H., Wang, Q., Huang, Y., Li, J., Zhou, Z.
Biochemical characterization and crystal structure of a GH10 xylanase from termite gut bacteria reveal a novel structural feature and significance of its bacterial Ig-like domain.,Han Q, Liu N, Robinson H, Cao L, Qian C, Wang Q, Xie L, Ding H, Wang Q, Huang Y, Li J, Zhou Z Biotechnol Bioeng. 2013 Jun 21. doi: 10.1002/bit.24982. PMID:23794438<ref>PMID:23794438</ref>


Description: Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hu8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Globitermes brachycerastes]]
[[Category: Large Structures]]
[[Category: Cao L]]
[[Category: Ding H]]
[[Category: Han Q]]
[[Category: Huang Y]]
[[Category: Li J]]
[[Category: Liu N]]
[[Category: Qian C]]
[[Category: Robinson H]]
[[Category: Wang Q]]
[[Category: Xie L]]
[[Category: Zhou Z]]
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