1ht9: Difference between revisions

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[[Image:1ht9.png|left|200px]]


{{STRUCTURE_1ht9| PDB=1ht9 | SCENE= }}
==DOMAIN SWAPPING EF-HANDS==
<StructureSection load='1ht9' size='340' side='right'caption='[[1ht9]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ht9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HT9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ht9 OCA], [https://pdbe.org/1ht9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ht9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ht9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ht9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1ht9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ht9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydrophobic cluster, in a region linking the EF-hands, promotes the conversion of monomers to 3D domain-swapped dimers. We propose a mechanism by which domain swapping takes place via the apo form of calbindin D(9k). Once formed, the calbindin D(9k) dimers are remarkably stable, as with even larger misfolded aggregates like amyloids. Thus calbindin D(9k) dimers cannot be converted to monomers by dilution. However, heating can be used for conversion, indicating high energy barriers separating monomers from dimers.


===DOMAIN SWAPPING EF-HANDS===
An extended hydrophobic core induces EF-hand swapping.,Hakansson M, Svensson A, Fast J, Linse S Protein Sci. 2001 May;10(5):927-33. PMID:11316872<ref>PMID:11316872</ref>


{{ABSTRACT_PUBMED_11316872}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ht9" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[1ht9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA].
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:011316872</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Fast, J.]]
[[Category: Large Structures]]
[[Category: Hakansson, M.]]
[[Category: Fast J]]
[[Category: Linse, S.]]
[[Category: Hakansson M]]
[[Category: Svensson, A L.]]
[[Category: Linse S]]
[[Category: Calbindin d9k]]
[[Category: Svensson AL]]
[[Category: Calcium binding]]
[[Category: Domain swapping]]
[[Category: Ef-hand]]
[[Category: Folding]]
[[Category: Metal binding protein]]
Retrieved from "https://proteopedia.org/1ht9"