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[[Image:1r9p.gif|left|200px]]<br /><applet load="1r9p" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1r9p" />
'''Solution NMR Structure Of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24.'''<br />


==Overview==
==Solution NMR Structure Of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24.==
IscU is a highly conserved protein that serves as the scaffold for IscS-mediated assembly of iron-sulfur ([Fe-S]) clusters. We report the NMR solution structure of monomeric Haemophilus influenzae IscU with zinc bound at the [Fe-S] cluster assembly site. The compact core of the globular structure has an alpha-beta sandwich architecture with a three-stranded antiparallel beta-sheet and four alpha-helices. A nascent helix is located N-terminal to the core structure. The zinc is ligated by three cysteine residues and one histidine residue that are located in and near conformationally dynamic loops at one end of the IscU structure. Removal of the zinc metal by chelation results in widespread loss of structure in the apo form. The zinc-bound IscU may be a good model for iron-loaded IscU and may demonstrate structural features found in the [Fe-S] cluster bound form. Structural and functional similarities, genomic context in operons containing other homologous genes, and distributions of conserved surface residues support the hypothesis that IscU protein domains are homologous (i.e. derived from a common ancestor) with the SufE/YgdK family of [Fe-S] cluster assembly proteins.
<StructureSection load='1r9p' size='340' side='right'caption='[[1r9p]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1r9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9P FirstGlance]. <br>
1R9P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9P OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9p OCA], [https://pdbe.org/1r9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9p RCSB], [https://www.ebi.ac.uk/pdbsum/1r9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9p ProSAT], [https://www.topsan.org/Proteins/NESGC/1r9p TOPSAN]</span></td></tr>
Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site., Ramelot TA, Cort JR, Goldsmith-Fischman S, Kornhaber GJ, Xiao R, Shastry R, Acton TB, Honig B, Montelione GT, Kennedy MA, J Mol Biol. 2004 Nov 19;344(2):567-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15522305 15522305]
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISCU_HAEIN ISCU_HAEIN] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9p_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r9p ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Acton, T B.]]
[[Category: Acton TB]]
[[Category: Cort, J R.]]
[[Category: Cort JR]]
[[Category: Kennedy, M A.]]
[[Category: Kennedy MA]]
[[Category: Montelione, G T.]]
[[Category: Montelione GT]]
[[Category: NESG, Northeast Structural Genomics Consortium.]]
[[Category: Ramelot TA]]
[[Category: Ramelot, T A.]]
[[Category: Shastry R]]
[[Category: Shastry, R.]]
[[Category: Xiao R]]
[[Category: Xiao, R.]]
[[Category: ZN]]
[[Category: 3beta strands]]
[[Category: 4 alpha helixes]]
[[Category: iron-sulfur cluster binding]]
[[Category: nesg]]
[[Category: northeast structural genomics consortium]]
[[Category: protein structure initiative]]
[[Category: psi-2]]
[[Category: structural genomics]]
[[Category: three conserved cys]]
[[Category: zinc binding]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:29 2008''
Retrieved from "https://proteopedia.org/1r9p"