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[[Image:1rh5.gif|left|200px]]<br /><applet load="1rh5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1rh5, resolution 3.20&Aring;" />
'''The structure of a protein conducting channel'''<br />


==Overview==
==The structure of a protein conducting channel==
A conserved heterotrimeric membrane protein complex, the Sec61 or SecY complex, forms a protein-conducting channel, allowing polypeptides to be transferred across or integrated into membranes. We report the crystal structure of the complex from Methanococcus jannaschii at a resolution of 3.2 A. The structure suggests that one copy of the heterotrimer serves as a functional translocation channel. The alpha-subunit has two linked halves, transmembrane segments 1-5 and 6-10, clamped together by the gamma-subunit. A cytoplasmic funnel leading into the channel is plugged by a short helix. Plug displacement can open the channel into an 'hourglass' with a ring of hydrophobic residues at its constriction. This ring may form a seal around the translocating polypeptide, hindering the permeation of other molecules. The structure also suggests mechanisms for signal-sequence recognition and for the lateral exit of transmembrane segments of nascent membrane proteins into lipid, and indicates binding sites for partners that provide the driving force for translocation.
<StructureSection load='1rh5' size='340' side='right'caption='[[1rh5]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1rh5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh5 OCA], [https://pdbe.org/1rh5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh5 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SECY_METJA SECY_METJA] The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/1rh5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rh5 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1RH5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH5 OCA].
*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
X-ray structure of a protein-conducting channel., Van den Berg B, Clemons WM Jr, Collinson I, Modis Y, Hartmann E, Harrison SC, Rapoport TA, Nature. 2004 Jan 1;427(6969):36-44. Epub 2003 Dec 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14661030 14661030]
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Protein complex]]
[[Category: Clemons Jr WM]]
[[Category: Berg, B van den.]]
[[Category: Collinson I]]
[[Category: Collinson, I.]]
[[Category: Harrison SC]]
[[Category: Harrison, S C.]]
[[Category: Hartmann E]]
[[Category: Hartmann, E.]]
[[Category: Modis Y]]
[[Category: Jr., W M.Clemons.]]
[[Category: Rapoport TA]]
[[Category: Modis, Y.]]
[[Category: Van den Berg B]]
[[Category: Rapoport, T A.]]
[[Category: membrane protein]]
[[Category: protein channels]]
[[Category: protein translocation]]
[[Category: secy]]
 
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Retrieved from "https://proteopedia.org/1rh5"