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| [[Image:1rw3.gif|left|200px]]<br /><applet load="1rw3" size="350" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[4mh8]] This PDB entry is obsolete and replaced by 4mh8 |
| caption="1rw3, resolution 3.0Å" />
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| '''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''<br />
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| ==Overview==
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| Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.
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| ==About this Structure==
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| 1RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
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| ==Reference==
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| The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus., Das D, Georgiadis MM, Structure. 2004 May;12(5):819-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15130474 15130474]
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| [[Category: Moloney murine leukemia virus]]
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| [[Category: Single protein]]
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| [[Category: Das, D.]]
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| [[Category: Georgiadis, M M.]]
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| [[Category: reverse transcriptase]]
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| [[Category: rna and dna dependent dna polymerase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:08 2008''
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