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| [[Image:1s1i.gif|left|200px]]<br /><applet load="1s1i" size="350" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[4v4b]] This PDB entry is obsolete and replaced by 4v4b |
| caption="1s1i" />
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| '''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.'''<br />
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| ==Overview==
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| An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
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| ==About this Structure==
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| 1S1I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1I OCA].
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| ==Reference==
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| Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation., Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jorgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J, EMBO J. 2004 Mar 10;23(5):1008-19. Epub 2004 Feb 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14976550 14976550]
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| [[Category: Protein complex]]
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| [[Category: Saccharomyces cerevisiae]]
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| [[Category: Andersen, G R.]]
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| [[Category: Ballesta, J P.G.]]
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| [[Category: Beckmann, R.]]
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| [[Category: Frank, J.]]
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| [[Category: Gomez-Lorenzo, M G.]]
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| [[Category: Grassucci, R A.]]
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| [[Category: Jorgensen, R.]]
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| [[Category: Penczek, P A.]]
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| [[Category: Spahn, C M.]]
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| [[Category: 60s ribosomal subunit]]
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| [[Category: 80s ribosome]]
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| [[Category: cryo-em]]
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| [[Category: eef2]]
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| [[Category: sordarin]]
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| [[Category: trna translocation]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:52 2008''
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This PDB entry is obsolete and replaced by 4v4b
