|
|
| (36 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| {{STRUCTURE_3han| PDB=3han | SIZE=400| SCENE= |right|CAPTION=Bacteriorhodopsin complex with retinal, [[3han]] }}
| | <StructureSection load='' size='350' side='right' scene='46/463242/Cv/4' caption='Bacteriorhodopsin complex with retinal, [[3han]]'> |
| | __TOC__ |
| | == Function == |
|
| |
|
| '''Bacteriorhodopsin''' (Br) is a membrane protein in Archaea which moves protons across the cell membrane. Br is composed of 6 α-helical elements each containing a retinal molecule. The retinal changes its conformation upon binding of a proton, causing the Br to change conformation and pump the proton. | | '''Bacteriorhodopsin''' (Br) is a membrane protein in Archaea which moves protons across the cell membrane<ref>PMID:28008064</ref>. See also [[Bacteriorhodopsin (Hebrew)]].<br /> |
| | '''Halorhodopsin''' uses light energy to pump chloride through biological membranes in Haloarchaea<ref>PMID:10827943</ref>.<br /> |
| | '''Sensory rhodopsin''' uses light energy to pump calcium ions through biological membranes. They are ubiquitous and use retinal as their chromophore<ref>PMID:17005405</ref>.<br /> |
| | '''Xanthorhodopsin''' is a light-driven proton pump which uses the carotenoid salinixanthin as a second chromophore<ref>PMID:18515067</ref>.<br /> |
| | '''Archaerhodopsin''' responds to yellow or green light by pumping protons out of cells and are used as optogenetic tools<ref>PMID:27524609</ref>.<br /> |
| | '''Proteorhodopsins''' are the most abundant retinal-based photoreceptors. It is found in many marine bacteria<ref>PMID:24060527</ref>.<br /> |
| | '''Deltarhodopsin''' is a light-driven proton pump found in the archaebacteria ''Halloterrigena turkmenica''<ref>PMID:23567447</ref>.<br /> |
| | '''Xenorhodopsin''' is an enigmatic new class of microbial rhodopsins<ref>PMID:21985229</ref>.<br /> |
|
| |
|
| {{TOC limit|limit=2}}
| | See also [[Rhodopsin]] |
|
| |
|
| == 3D Structures of bacteriorhodopsin == | | == Structural highlights == |
|
| |
|
| ''Update May 2012'' | | Br is composed of 6 α-helical elements each containing a retinal molecule. The retinal changes its ground state conformation upon binding of a proton, causing the Br to change conformation to the activated state and pump the proton. <scene name='46/463242/Cv/6'>The retinal interacts predominantly with hydrophobic and aromatic residues</scene> <ref>PMID:19603754</ref> ({{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}). |
|
| |
|
| [[3ns0]], [[3nsb]], [[3mbv]], [[1x0s]], [[1x0i]], [[1x0k]], [[1xji]], [[1iw6]] – HsBr residues 14-261 + lipid + retinal – ''Halobacterium salinarum''<br />
| | == 3D Structures of bacteriorhodopsin == |
| [[2wjk]], [[2wjl]] - HsBr residues 14-261 (mutant) + lipid + retinal<br />
| | [[Bacteriorhodopsin 3D structures]] |
| [[3han]], [[2hao]], [[3hap]], [[3haq]], [[3har]], [[3has]], [[3coc]], [[3cod]], [[1tn5]], [[1tn0]], [[1s51]], [[1s52]], [[1s53]], [[1s54]], [[1q5i]], [[1q5j]], [[1pxr]], [[1pxs]], [[3hao]], [[3utv]], [[3utw]], [[3utx]], [[3uty]] - HsBr residues 14-261 (mutant) + retinal<br />
| |
| [[2ntu]], [[1py6]], [[1fbb]], [[1fbk]], [[1brx]], [[1ap9]], [[1at9]] - HsBr residues 14-261 + retinal<br />
| |
| [[2i1x]], [[1kme]], [[1qm8]], [[1bm1]], [[1brr]], [[2brd]] - HsBr residues 14-261 + lipid + retinal<br />
| |
| [[2at9]] - HsBr residues 14-261 + lipid + retinal – Cryo EM<br />
| |
| [[1brd]] - HsBr residues 14-261 + retinal – Cryo EM<br />
| |
| [[1s8j]], [[3t45]] - HsBr residues 14-261 (mutant) + lipid + NO3 + retinal<br />
| |
| [[1mgy]] - HsBr residues 14-261 (mutant) + lipid + Br + retinal<br />
| |
| [[1s8l]] - HsBr residues 14-261 (mutant) + lipid + retinal<br />
| |
| [[1qhj]] - HsBr residues 14-261 + phytanyl derivavative + retinal<br />
| |
| [[1r84]], [[1r2n]] - HsBr residues 1-232 + retinal – NMR<BR />
| |
| [[1bct]] - HsBr residues 163-231 – NMR<BR />
| |
| [[1bha]], [[1bhb]] - HsBr residues 1-71 - NMR<BR />
| |
| [[1l0m]] – Br (mutant) – ''Halobacterium halobium'' - NMR<BR />
| |
| | |
| ===Bacteriorhodopsin intermediates===
| |
| | |
| [[1f50]], [[1c8r]] - HsBr-BR residues 18-244 (mutant) + lipid + retinal<br />
| |
| [[1c3w]] - HsBr-BR residues 5-231 + lipid + retinal<br />
| |
| [[2zfe]] – HsBr-K residues 14-261 + lipid + retinal<br />
| |
| [[1m0k]], [[1m0l]], [[1ixf]] - HsBr-K residues 1-261 + lipid + retinal<br />
| |
| [[1m0m]] - HsBr-M1 residues 1-261 + lipid + retinal<br />
| |
| [[1e0p]], [[2ntw]], [[1vjm]] - HsBr-L residues 18-245 + retinal<br />
| |
| [[1ucq]], [[1iw9]], [[1o0a]] – HsBr-L residues 14-261 + lipid + retinal<br />
| |
| [[1kg8]], [[1kg9]], [[1kgb]], [[1dze]], [[1cwq]], [[2zzl]], [[2i20]], [[2i21]], [[1p8h]] - HsBr-M residues 13-243 + lipid + retinal<br />
| |
| [[1f4z]], [[1c8s]], [[1p8i]] - HsBr-M residues 18-244 (mutant) + lipid + retinal<br />
| |
| [[1p8u]] – HsBr-N residues 14-261 (mutant) + lipid + retinal<br />
| |
| [[1jv6]], [[1jv7]] - HsBr-O residues 1-261 (mutant) + lipid + retinal<br />
| |
| [[1qko]], [[1qkp]] - HsBr residues 14-261 + retinal<br />
| |
| | |
| ===Halorhodopsin===
| |
| | |
| [[3abw]], [[3a7k]] – NpBr+ N3 + bacterioruberin + detergent + retinal – ''Natronomonas pharaonis''<br /> | |
| [[2jaf]], [[2jag]], [[1e12]] - HsBr (mutant) + lipid+ retinal<br />
| |
| | |
| ===Sensory rhodopsin II===
| |
| | |
| [[2ksy]] – NpBr – NMR<BR />
| |
| [[2f93]], [[2f95]], [[1h2s]] - BpBr + Br transducer + detergent + retinal<br />
| |
| [[1gu8]], [[1gue]], [[1h68]], [[1jgj]] - NpBr + retinal<br />
| |
| [[1xio]] - Br + detergent + retinal - ''Anabaena''
| |
| | |
| ===Xanthorhodopsin===
| |
| | |
| [[3ddl]] – Br + detergent + salinixanthin + retinal – ''Salinibacter ruber''
| |
| | |
| ===Archaerhodopsin===
| |
| | |
| [[2ei4]], [[2z55]] – HaBr + bacterioruberin + detergent + retinal – ''Halobacterium''<br />
| |
| [[1vgo]] - HaBr + detergent + retinal<br />
| |
| [[1uaz]] - HaBr + retinal<br />
| |
|
| |
|
| | </StructureSection> |
|
| |
|
| ==See Also== | | ==See Also== |
| Line 67: |
Line 27: |
| *[[G protein-coupled receptor]] | | *[[G protein-coupled receptor]] |
|
| |
|
| | | == References == |
| | | <references/> |
| | |
| | |
| | |
| | |
| | |
| [[he:Bacteriorhodopsin_(Hebrew)]] | | [[he:Bacteriorhodopsin_(Hebrew)]] |
|
| |
|
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
